Any feedback?
Literature summary extracted from
- The expression, purification and activity analysis of Francisella tularensis citrulline ureidase in Escherichia coli (2015), J. Appl. Biomed., 13, 189-194 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.5.1.20 | expression in Escherichia coli. The soluble and active recombinant enzyme is expressed in the periplasmic space with the vector pET-22b | Francisella tularensis subsp. tularensis |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.5.1.20 | periplasm | - |
Francisella tularensis subsp. tularensis | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.20 | 37000 | - |
SDS-PAGE | Francisella tularensis subsp. tularensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.20 | L-citrulline + H2O | Francisella tularensis subsp. tularensis | - |
L-ornithine + CO2 + NH3 | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.5.1.20 | Francisella tularensis subsp. tularensis | Q5NHL7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.5.1.20 | recombinant His-tagged enzyme | Francisella tularensis subsp. tularensis |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.5.1.20 | 20 | 24 | 30°C, pH 6.5 | Francisella tularensis subsp. tularensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.5.1.20 | L-citrulline + H2O | - |
Francisella tularensis subsp. tularensis | L-ornithine + CO2 + NH3 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.5.1.20 | ? | x * 37000, SDS-PAGE | Francisella tularensis subsp. tularensis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.5.1.20 | citrulline ureidase | - |
Francisella tularensis subsp. tularensis |
| 3.5.1.20 | CTU | - |
Francisella tularensis subsp. tularensis |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
