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Literature summary extracted from

  • Cai, X.; Jiang, H.; Zhang, T.; Jiang, B.; Mu, W.; Miao, M.
    Thermostability and specific-activity enhancement of an arginine deiminase from Enterococcus faecalis SK23.001 via semirational design for L-citrulline production (2018), J. Agric. Food Chem., 66, 8841-8850 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.3.6 synthesis L-citrulline can be enzymatically produced by arginine deiminase Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.6 expression in Escherichia coli BL21(DE3) Enterococcus faecalis

Protein Variants

EC Number Protein Variants Comment Organism
3.5.3.6 F269P the conversion rate of L-arginine to L-citrulline at 45°C is 93.2% compared to wild-type enzyme Enterococcus faecalis
3.5.3.6 G292C the conversion rate of L-arginine to L-citrulline at 45°C is 96.0% compared to wild-type enzyme Enterococcus faecalis
3.5.3.6 G292D the conversion rate of L-arginine to L-citrulline at 45°C is 97.5% compared to wild-type enzyme Enterococcus faecalis
3.5.3.6 G292P the conversion rate of L-arginine to L-citrulline at 45°C is 96.5% compared to wild-type enzyme Enterococcus faecalis
3.5.3.6 R15K the conversion rate of L-arginine to L-citrulline at 45°C is 95.8% compared to wild-type enzyme Enterococcus faecalis
3.5.3.6 R15K/F269Y/G292P the triple-site mutant enzyme displays a 2.5fold higher specific enzyme activity (333 U/mg), a lower Km value of 6.4 mM, and a 6.1fold longer half-life (t1/2(45°C): 86.7 min) than wild-type enzyme. The conversion rate of L-arginine to L-citrulline at 45°C is 97.4% compared to wild-type enzyme Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.6 6.4
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/F269Y/G292P Enterococcus faecalis
3.5.3.6 6.7
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/G292P Enterococcus faecalis
3.5.3.6 9.3
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/F269Y Enterococcus faecalis
3.5.3.6 10.1
-
 L-arginine pH 6.0, 45°C, wild-type enzyme Enterococcus faecalis
3.5.3.6 11.6
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K Enterococcus faecalis
3.5.3.6 12.5
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/G264P Enterococcus faecalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.5.3.6 L-arginine + H2O Enterococcus faecalis
-
 L-citrulline + NH3
-
 ?
3.5.3.6 L-arginine + H2O Enterococcus faecalis SK23.001
-
 L-citrulline + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.6 Enterococcus faecalis A0A3G2YMH7
-
-
3.5.3.6 Enterococcus faecalis SK23.001 A0A3G2YMH7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.3.6 126.5
-
 substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/F269Y Enterococcus faecalis
3.5.3.6 131.2
-
 substrate: L-arginine, pH 6.0, 45°C, wild-type enzyme Enterococcus faecalis
3.5.3.6 134.7
-
 substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/G264P Enterococcus faecalis
3.5.3.6 143.2
-
 substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K Enterococcus faecalis
3.5.3.6 296.8
-
 substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/G292P Enterococcus faecalis
3.5.3.6 332.8
-
 substrate: L-arginine, pH 6.0, 45°C, mutant enzyme R15K/F269Y/G292P Enterococcus faecalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.6 L-arginine + H2O
-
 Enterococcus faecalis L-citrulline + NH3
-
 ?
3.5.3.6 L-arginine + H2O
-
 Enterococcus faecalis SK23.001 L-citrulline + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.6 ADI
-
 Enterococcus faecalis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.3.6 55
-
-
 Enterococcus faecalis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.3.6 45
-
 t1/2: 14.3 (wild-type enzyme), t1/2: 14.5 (mutant enzyme R15K), t1/2: 14.9 (mutant enzyme R15K/G264P), t1/2: 48.0 (mutant enzyme R15K/F269Y), t1/2: 82.5 (mutant enzyme R15K/G292P), t1/2: 86.7 min (mutant enzyme R15K/F269Y/G292P) Enterococcus faecalis
3.5.3.6 60
-
 t1/2: 12 (wild-type enzyme) Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.3.6 268
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K Enterococcus faecalis
3.5.3.6 291
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/G264P Enterococcus faecalis
3.5.3.6 309
-
 L-arginine pH 6.0, 45°C, wild-type enzyme Enterococcus faecalis
3.5.3.6 342
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/F269Y Enterococcus faecalis
3.5.3.6 365
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/F269Y/G292P Enterococcus faecalis
3.5.3.6 371
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/G292P Enterococcus faecalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.6 7.5
-
-
 Enterococcus faecalis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.3.6 23.1
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K Enterococcus faecalis
3.5.3.6 23.3
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/G264P Enterococcus faecalis
3.5.3.6 30.6
-
 L-arginine pH 6.0, 45°C, wild-type enzyme Enterococcus faecalis
3.5.3.6 36.8
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/F269Y Enterococcus faecalis
3.5.3.6 55.4
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/G292P Enterococcus faecalis
3.5.3.6 57
-
 L-arginine pH 6.0, 45°C, mutant enzyme R15K/F269Y/G292P Enterococcus faecalis