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Literature summary extracted from

  • Alhelli, A.; Manap, M.; Mohammed, A.; Mirhosseini, H.; Suliman, E.; Shad, Z.; Mohammed, N.; Hussin, A.
    Response surface methodology modelling of an aqueous two-phase system for purification of protease from Penicillium candidum (PCA 1/TT031) under solid state fermentation and its biochemical characterization (2016), Int. J. Mol. Sci., 17, 1872 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.4.23.20 food industry the enzyme is used in the dairy industry such as in accelerated cheese ripening Penicillium candidum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.20 Ca2+ 23% residual activity at 10 mM Penicillium candidum
3.4.23.20 EDTA 7% residual activity at 10 mM Penicillium candidum
3.4.23.20 Fe3+ complete inhibition at 10 mM Penicillium candidum
3.4.23.20 K+ 85% residual activity at 10 mM Penicillium candidum
3.4.23.20 Mg2+ 31% residual activity at 10 mM Penicillium candidum
3.4.23.20 Mn2+ 98% residual activity at 10 mM Penicillium candidum
3.4.23.20 additional information not inhibited by phenylmethylsulfonyl fluoride Penicillium candidum
3.4.23.20 SDS 33% residual activity at 10 mM Penicillium candidum
3.4.23.20 Zn2+ 92% residual activity at 10 mM Penicillium candidum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.23.20 K+ 112% activity at 5 mM Penicillium candidum
3.4.23.20 Mn2+ 110% activity at 5 mM Penicillium candidum
3.4.23.20 Na+ 136% activity at 5 mM Penicillium candidum
3.4.23.20 Zn2+ 122% activity at 5 mM Penicillium candidum

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.20 Penicillium candidum
-
-
-
3.4.23.20 Penicillium candidum PCA 1/TT031
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.20 aqueous two-phase system system using 9% (w/v) PEG 8000, 5.2% (w/v) NaCl, and 15.9% (w/v) sodium citrate Penicillium candidum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.20 azocasein + H2O
-
 Penicillium candidum ?
-
 ?
3.4.23.20 azocasein + H2O
-
 Penicillium candidum PCA 1/TT031 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.20 ? x * 100000-140000, SDS-PAGE Penicillium candidum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.20 50
-
-
 Penicillium candidum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.23.20 4 50 the enzyme retains more than 80% of its initial activity at 4-50 °C. The activity is reduced to 52% at 60°C Penicillium candidum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.20 6
-
-
 Penicillium candidum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.20 5 7 more than 77% activity between pH 5.0 and 7.0 Penicillium candidum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.20 8 9 the enzyme retains less than 35% activity after 1 h at pH 8.0-9.0 Penicillium candidum