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Literature summary extracted from

  • Sao Paulo Barretto Miranda, I.K.; Fontes Suzart Miranda, A.; Souza, F.V.; Vannier-Santos, M.A.; Pirovani, C.P.; Pepe, I.M.; Rodowanski, I.J.; Ferreira, K.T.; Mendes Souza Vaz, L.; de Assis, S.A.
    The biochemical characterization, stabilization studies and the antiproliferative effect of bromelain against B16F10 murine melanoma cells (2017), Int. J. Food Sci. Nutr., 68, 442-454 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.32 pharmacology bromelain is pharmacologically active against B16F10 melanoma cells with complete inhibition of tumor cell proliferation in vitro Ananas comosus
3.4.22.33 pharmacology bromelain is pharmacologically active against B16F10 melanoma cells with complete inhibition of tumor cell proliferation in vitro Ananas comosus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.32 additional information stability of bromelain in the presence of EDTA and sodium benzoate Ananas comosus
3.4.22.33 additional information stability of bromelain in the presence of EDTA and sodium benzoate; stability of bromelain in the presence of EDTA and sodium benzoate Ananas comosus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.32 Ananas comosus P14518 an unexplored French Guiana variety, collected in 1993
-
3.4.22.32 Ananas comosus AGB 772 P14518 an unexplored French Guiana variety, collected in 1993
-
3.4.22.33 Ananas comosus O23791 pulp enzyme; an unexplored French Guiana variety, collected in 1993
-
3.4.22.33 Ananas comosus O23801 peel enzyme; an unexplored French Guiana variety, collected in 1993
-
3.4.22.33 Ananas comosus AGB 772 O23791 pulp enzyme; an unexplored French Guiana variety, collected in 1993
-
3.4.22.33 Ananas comosus AGB 772 O23801 peel enzyme; an unexplored French Guiana variety, collected in 1993
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.32 leaf
-
 Ananas comosus
-
3.4.22.32 additional information analysis of bromelain activity in different Ananas comosus parts and tissues, overview Ananas comosus
-
3.4.22.32 stem
-
 Ananas comosus
-
3.4.22.33 exocarp
-
 Ananas comosus
-
3.4.22.33 additional information analysis of bromelain activity in different Ananas comosus parts and tissues, overview Ananas comosus
-
3.4.22.33 plant crown
-
 Ananas comosus
-
3.4.22.33 pulp
-
 Ananas comosus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.32 432.98
-
 extracted stem bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus
3.4.22.32 859.44
-
 extracted leaf bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus
3.4.22.33 1050.57
-
 extracted crown bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus
3.4.22.33 1365.77
-
 extracted peel bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus
3.4.22.33 1519.85
-
 extracted pulp bromelain, pH 6.0, 37°C, with substrate casein Ananas comosus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.32 casein + H2O
-
 Ananas comosus ?
-
 ?
3.4.22.32 casein + H2O
-
 Ananas comosus AGB 772 ?
-
 ?
3.4.22.33 casein + H2O
-
 Ananas comosus ?
-
 ?
3.4.22.33 casein + H2O
-
 Ananas comosus AGB 772 ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.32 60
-
 stem enzyme Ananas comosus
3.4.22.32 65
-
 leaf enzyme Ananas comosus
3.4.22.33 50 60 pulp enzyme Ananas comosus
3.4.22.33 50 70 crown enzyme Ananas comosus
3.4.22.33 60 80 peel enzyme Ananas comosus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.22.32 60 80 bromelain from the leaves shows higher activity between 60 and 80°C Ananas comosus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.32 6
-
 leaf (first peak) and stem enzymes Ananas comosus
3.4.22.32 8
-
 leaf enzyme, second peak Ananas comosus
3.4.22.33 6
-
-
 Ananas comosus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.22.32 6 8 leaves present two peaks at pH 6.0 and pH 8.0 Ananas comosus

General Information

EC Number General Information Comment Organism
3.4.22.32 physiological function antiproliferative effect of bromelain from different tissues against B16F10 murine melanoma cells, overview Ananas comosus
3.4.22.33 physiological function antiproliferative effect of bromelain from different tissues against B16F10 murine melanoma cells, overview Ananas comosus