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Literature summary extracted from
- Kinetics and conformational stability studies of recombinant leucine aminopeptidase (2014), Int. J. Biol. Macromol., 64, 306-312 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | synthesis | leucine aminopeptidase from Vibrio proteolyticus is a broad specificity N-terminal aminopeptidase that is widely used in pharmaceutical processes where the removal of N-terminal residues in recombinant proteins is required | Vibrio proteolyticus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | recombinant expression of His6-tagged enzyme | Vibrio proteolyticus |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.4.11.10 | urea-caused unfolding transitions of rLAP as a function of urea concentration are monitored by circular dichroism (CD) and fluorescence (FL) spectroscopy exhibiting single transitions by both techniques. Free energy change for unfolding measured by CD and FL spectroscopy are 2.8 and 3.7 kcal/mol, respectively | Vibrio proteolyticus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.10 | DTT | complete inhibition at 20 mM | Vibrio proteolyticus |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.10 | additional information | - |
additional information | thermodynamics and Michaelis-Menten kinetics, overview | Vibrio proteolyticus | |
| 3.4.11.10 | 0.245 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C) | Vibrio proteolyticus | |
| 3.4.11.10 | 0.311 | - |
L-Leu-4-nitroanilide | pH 8.0, 70°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C) | Vibrio proteolyticus | |
| 3.4.11.10 | 0.902 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant His6-tagged enzyme, without preincubation | Vibrio proteolyticus | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.11.10 | Zn2+ | required, 2 Zn2+ per active site | Vibrio proteolyticus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.11.10 | Vibrio proteolyticus | Q01693 | - |
- |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 3.4.11.10 | activity gain of rLAP refolded after reversible denaturation by urea, at 37°C, monitoring of the secondary structure changes | Vibrio proteolyticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.11.10 | L-Leu-4-nitroanilide + H2O | - |
Vibrio proteolyticus | L-Leu + 4-nitroaniline | - |
? | |
| 3.4.11.10 | additional information | recombinant enzyme rLAP is selective and specific to remove N-terminal groups from amino acids | Vibrio proteolyticus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | AVP | - |
Vibrio proteolyticus |
| 3.4.11.10 | leucine aminopeptidase | - |
Vibrio proteolyticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 70 | - |
- |
Vibrio proteolyticus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.10 | 0.817 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant His6-tagged enzyme, without preincubation | Vibrio proteolyticus | |
| 3.4.11.10 | 15.8 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C) | Vibrio proteolyticus | |
| 3.4.11.10 | 48.45 | - |
L-Leu-4-nitroanilide | pH 8.0, 70°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C) | Vibrio proteolyticus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.4.11.10 | 8 | - |
assay at | Vibrio proteolyticus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.11.10 | additional information | temperature-dependent UV-CD spectra and fluorescent measurements | Vibrio proteolyticus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.11.10 | 0.91 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant His6-tagged enzyme, without preincubation | Vibrio proteolyticus | |
| 3.4.11.10 | 64.5 | - |
L-Leu-4-nitroanilide | pH 8.0, 37°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C) | Vibrio proteolyticus | |
| 3.4.11.10 | 155.8 | - |
L-Leu-4-nitroanilide | pH 8.0, 70°C, recombinant His6-tagged enzyme, with preincubation (72 h of incubation at 37°C) | Vibrio proteolyticus | |
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