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Literature summary extracted from
- Jmjd1a and Jmjd2c histone H3 Lys 9 demethylases regulate self-renewal in embryonic stem cells (2007), Genes Dev., 21, 2545-2557 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.65 | gene KDM3A, cloning of the Jmjd1a intronic DNA containing the Oct4-binding sites upstream of or downstream from a luciferase reporter to test for enhancer activity, robust enhancer activity is observed when the constructs is transfected into ES cells, real-time PCR expression analysis | Mus musculus |
| 1.14.11.66 | gene KDM4C, cloning of the Jmjd2c intronic DNA containing these Oct4-binding sites upstream of or downstream from a luciferase reporter to test for enhancer activity, robust enhancer activity is observed when the constructs is transfected into embryonic stem cells, real-time PCR expression analysis | Mus musculus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 1.14.11.65 | additional information | construction of Jmjd1a knockout embryonic stem cells by RNAi assay, Jmjd1a depletion leads to embryonic stem cell differentiation, which is accompanied by a reduction in the expression of embryonic stem cell-specific genes and an induction of lineage marker genes. The same mutations that disrupt the in vitro Oct4/DNA interactions also abolish the enhancer activities. Knockdown of Jmjd1a does not appreciably affect Jmjd2c and vice versa | Mus musculus |
| 1.14.11.66 | additional information | construction of Jmjd2c knockout embryonic stem cells by RNAi assay, Jmjd2c depletion leads to embryonic stem cell differentiation, which is accompanied by a reduction in the expression of embryonic stem cell-specific genes and an induction of lineage marker genes. The same mutations that disrupt the in vitro Oct4/DNA interactions also abolished the enhancer activities. Knockdown of Jmjd1a does not appreciably affect Jmjd2c and vice versa | Mus musculus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.11.65 | chromatin | - |
Mus musculus | 785 | - |
| 1.14.11.65 | nucleus | - |
Mus musculus | 5634 | - |
| 1.14.11.66 | chromatin | - |
Mus musculus | 785 | - |
| 1.14.11.66 | nucleus | - |
Mus musculus | 5634 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.65 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Mus musculus | - |
[histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? |  |
| 1.14.11.65 | [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 | Mus musculus | - |
[histone H3]-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.66 | [histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Mus musculus | - |
[histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.66 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Mus musculus | - |
[histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.65 | Mus musculus | Q6PCM1 | - |
- |
| 1.14.11.66 | Mus musculus | Q8VCD7 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.65 | embryonic stem cell | feeder-free E14 mouse ES cells | Mus musculus | - |
| 1.14.11.66 | embryonic stem cell | feeder-free E14 mouse ES cells | Mus musculus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.65 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Mus musculus | [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.65 | [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Mus musculus | [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.66 | additional information | the bifunctional enzyme is active on H3K9me3/me2 and H3K36me3/me2 (EC 1.14.11.69) substrates | Mus musculus | ? | - |
? | |
| 1.14.11.66 | [histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Mus musculus | [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.66 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Mus musculus | [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.65 | H3K9Me2 demethylase | - |
Mus musculus |
| 1.14.11.65 | histone H3 Lys 9 demethylase | - |
Mus musculus |
| 1.14.11.65 | JMJD1A | - |
Mus musculus |
| 1.14.11.65 | Kdm3a | - |
Mus musculus |
| 1.14.11.66 | H3K9Me3 demethylase | - |
Mus musculus |
| 1.14.11.66 | histone H3 Lys 9 demethylase | - |
Mus musculus |
| 1.14.11.66 | JMJD2C | - |
Mus musculus |
| 1.14.11.66 | Kdm4c | - |
Mus musculus |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 1.14.11.65 | Mus musculus | gene Jmjd1a is positively regulated by the ES cell transcription factor Oct4 | up |
| 1.14.11.66 | Mus musculus | gene Jmjd2c is positively regulated by the ES cell transcription factor Oct4 | up |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.65 | malfunction | Jmjd1a depletion leads to embryonic stem cell differentiation, which is accompanied by a reduction in the expression of embryonic stem cell-specific genes and an induction of lineage marker genes. The level of H3K9Me2, but not H3K9Me3, of total cell histone H3, is increased upon Jmjd1a knockdown. Knockdown of Jmjd1a does not appreciably affect Jmjd2c and vice versa | Mus musculus |
| 1.14.11.65 | metabolism | expression of histone H3 Lys 9 demethylases Jmjd1a and Jmjd2c (EC 1.14.11.66) is positively correlated with the pluripotent state of ES and iPS cells. Jmjd1a and Jmjd2c regulate the global levels of H3K9Me2 and H3K9Me3, respectively | Mus musculus |
| 1.14.11.65 | physiological function | Jmjd1a is involved in the reversal of H3K9Me2 of bulk chromatin in embryonic stem cells. Jmjd1a demethylates H3K9Me2 at the promoter regions of Tcl1, Tcfcp2l1, and Zfp57 and positively regulates the expression of these pluripotency-associated genes, detailed overview. The embryonic stem cell transcription circuitry is connected to chromatin modulation through H3K9 demethylation in pluripotent cells | Mus musculus |
| 1.14.11.66 | malfunction | Jmjd2c depletion leads to embryonic stem cell differentiation, which is accompanied by a reduction in the expression of embryonic stem cell-specific genes and an induction of lineage marker genes. The level of H3K36Me3 is not significantly affected by Jmjd2c depletion. Knockdown of Jmjd1a does not appreciably affect Jmjd2c and vice versa | Mus musculus |
| 1.14.11.66 | metabolism | expression of histone H3 Lys 9 demethylases Jmjd1a (EC 1.14.11.65) and Jmjd2c is positively correlated with the pluripotent state of ES and iPS cells. Jmjd1a and Jmjd2c regulate the global levels of H3K9Me2 and H3K9Me3, respectively | Mus musculus |
| 1.14.11.66 | physiological function | Jmjd2c acts as a positive regulator for Nanog, which encodes for a key transcription factor for self-renewal in ES cells. Jmjd2c is required to reverse the H3K9Me3 marks at the Nanog promoter region and consequently prevents transcriptional repressors HP1 and KAP1 from binding. Jmjd2c regulates expression of Nanog through demethylation of H3K9Me3, overview. The ES cell transcription circuitry is connected to chromatin modulation through H3K9 demethylation in pluripotent cells | Mus musculus |
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