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Literature summary extracted from

  • Du, H.; Pang, M.; Dong, Y.; Wu, Y.; Wang, N.; Liu, J.; Awan, F.; Lu, C.; Liu, Y.
    Identification and characterization of an Aeromonas hydrophila oligopeptidase gene pepF negatively related to biofilm formation (2016), Front. Microbiol., 7, 1497 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.B2 screening of a EZ-Tn5 transposon random mutant library and cloning of gene pepF, DNA and amino acid sequence determination and analysis, real-time quantitative PCR expression analysis Aeromonas hydrophila

Protein Variants

EC Number Protein Variants Comment Organism
3.4.24.B2 additional information identification of the TM90 mutant strain with defective pepF, phenotype, overview Aeromonas hydrophila

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.B2 Aeromonas hydrophila
-
-
-
3.4.24.B2 Aeromonas hydrophila NJ-35
-
-
-

Subunits

EC Number Subunits Comment Organism
3.4.24.B2 ? x * 68400, about, sequence calculation Aeromonas hydrophila

Synonyms

EC Number Synonyms Comment Organism
3.4.24.B2 PepF
-
 Aeromonas hydrophila

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.24.B2 Aeromonas hydrophila sequence calculation
-
 5.42

General Information

EC Number General Information Comment Organism
3.4.24.B2 evolution enzyme PepF is a metallopeptidase belonging to the M3 family Aeromonas hydrophila
3.4.24.B2 malfunction the mutant strain TM90, in which gene pepF, encoding oligopeptidase F, is disturbed, shows significant upregulation of biofilm formation compared to the parental strain. The TM90 colony phenotype is smaller, more transparent, and splendent. The adhesive ability of TM90 to HEP-G2 cells is significantly increased compared with the parental strain. Fifty percent lethal dose (LD50) determinations in zebrafish demonstrats that the enhanced-biofilm mutant TM90 is highly attenuated relative to the wild-type strain Aeromonas hydrophila
3.4.24.B2 physiological function Aeromonas hydrophila oligopeptidase F, encoded by gene pepF, is negatively involved in biofilm formation. Enzyme PepF might contribute to Aeromonas hydrophila virulence Aeromonas hydrophila