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Literature summary extracted from

  • Celias, D.P.; Corvo, I.; Silvane, L.; Tort, J.F.; Chiapello, L.S.; Fresno, M.; Arranz, A.; Motran, C.C.; Cervi, L.
    Cathepsin L3 from Fasciola hepatica induces NLRP3 inflammasome alternative activation in murine dendritic cells (2019), Front. Immunol., 10, 552 .
    View publication on PubMedView publication on EuropePMC

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.22.B62 extracellular the enzyme is secreted Fasciola hepatica
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Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.B62 Collagen + H2O Fasciola hepatica
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 ?
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 ?
3.4.22.B62 pro-interleukin-1beta + H2O Fasciola hepatica
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 interleukin-1beta + interleukin-1beta propeptide
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 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.B62 Fasciola hepatica B3TM67
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-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.B62 additional information cathepsin L3 (FhCL3) is expressed mainly in the juvenile or invasive stage Fasciola hepatica
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.B62 Collagen + H2O
-
 Fasciola hepatica ?
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 ?
3.4.22.B62 pro-interleukin-1beta + H2O
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 Fasciola hepatica interleukin-1beta + interleukin-1beta propeptide
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 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.B62 FhCL3
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 Fasciola hepatica

General Information

EC Number General Information Comment Organism
3.4.22.B62 physiological function in the case of the helminth trematode Fasciola hepatica, the secretion of different cathepsin L cysteine peptidases (FhCL) is crucial for the parasite survival. Among these enzymes, cathepsin L3 (FhCL3) is expressed mainly in the juvenile or invasive stage. The ability of FhCL3 to digest collagen is critical for intestinal tissue invasion during juvenile larvae migration. FhCL3 induces a non-canonical inflammasome activation in dendritic cells (DCs) of C57BL/6 mice, leading to interleukin (IL)-1beta and IL-18 production without a previous microbial priming. This activation is depending on the cysteine protease activity of FhCL3 and the NLRP3 receptor, but independent of caspase activation. FhCL3 is internalized by DCs, promoting pro-IL-1beta cleavage to its mature and biologically active form IL-1beta, which is released to the extracellular environment. The FhCL3-induced NLRP3 inflammasome activation conditions DCs to promote a singular adaptive immune response, characterized by increased production of IFN-gamma and IL-13. Due to the poor colocalization of FhCL3 with endosomes or lysosomes, the mechanism of endocytosis could be different from that used by FhCL1. The lack of involvement of NF-kkappaB in FhCL3-induced DCs activation, suggests that other/s transcription factor might be involved in the synthesis of pro-IL-1beta Fasciola hepatica