EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.1 | EDTA | 10 mM, 62% residual activity | Phaseolus vulgaris | |
3.5.1.1 | Hg2+ | 10 mM, 33% residual activity | Phaseolus vulgaris |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.1 | 6.72 | - |
L-asparagine | pH 8.0, 37°C | Phaseolus vulgaris |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.1 | Ca2+ | 10 mM, 110% of initial activity | Phaseolus vulgaris | |
3.5.1.1 | K+ | 10 mM, 150% of initial activity | Phaseolus vulgaris |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 79000 | - |
gel filtration | Phaseolus vulgaris |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.1 | Phaseolus vulgaris | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.1 | - |
Phaseolus vulgaris |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.5.1.1 | seed | - |
Phaseolus vulgaris | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 846 | - |
pH 8.0, 37°C | Phaseolus vulgaris |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.1 | L-asparagine + H2O | - |
Phaseolus vulgaris | L-aspartate + NH3 | - |
? | |
3.5.1.1 | additional information | poor substrates: D-asparagine, L-aspartic acid, and L-glutamic acid. No substrate: L-glutamine | Phaseolus vulgaris | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.1 | monomer | 1 * 79000, SDS-PAGE | Phaseolus vulgaris |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 37 | - |
- |
Phaseolus vulgaris |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 8 | - |
- |
Phaseolus vulgaris |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.1 | 6 | 9 | more than 50% of maximum activity | Phaseolus vulgaris |