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Literature summary extracted from

  • Vallee, B.; Riezman, H.
    Lip1p a novel subunit of acyl-CoA ceramide synthase (2005), EMBO J., 24, 730-741 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.297 genes LAC1, LAG1, and LIP1. LIP1 DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of all three subunits as N-terminally FLAG- or HA-tagged proteins in Saccharomyces cerevisiae strains Saccharomyces cerevisiae

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.297 australifungin
-
 Saccharomyces cerevisiae
2.3.1.297 fumonisin B1
-
 Saccharomyces cerevisiae

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.297 endoplasmic reticulum membrane Lac1p and Lag1p are integral membrane proteins with six to eight predicted transmembrane domains. Co-subunit Lip1 is also an integral membrane protein, with one transmembrane domain. Its short N-terminal part is cytoplasmic and not required for ceramide synthesis. Analysis of membrane topology of the heterotrimer for ceramide synthase catalytic activity, overview Saccharomyces cerevisiae 5789
-
2.3.1.297 microsome
-
 Saccharomyces cerevisiae
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA Saccharomyces cerevisiae
-
 N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA Saccharomyces cerevisiae RH4838
-
 N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA Saccharomyces cerevisiae
-
 N-cerotoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA Saccharomyces cerevisiae RH4838
-
 N-cerotoyl-D-sphinganine + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.297 Saccharomyces cerevisiae P28496 AND P38703 AND Q03579 encoding subunits Lac1p, Lag1p, and Lip1p
-
2.3.1.297 Saccharomyces cerevisiae RH4838 P28496 AND P38703 AND Q03579 encoding subunits Lac1p, Lag1p, and Lip1p
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.297 native enzyme 3420fold subunits from isolated digitonin-treated membranes by solubilization with 1% Triton X-100 and affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.297 20.68
-
 purified native enzyme, pH 6.8, 24°C Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA
-
 Saccharomyces cerevisiae N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + arachidoyl-CoA
-
 Saccharomyces cerevisiae RH4838 N-arachidoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA
-
 Saccharomyces cerevisiae N-cerotoyl-D-sphinganine + CoA
-
 ?
2.3.1.297 D-erythro-sphinganine + cerotoyl-CoA
-
 Saccharomyces cerevisiae RH4838 N-cerotoyl-D-sphinganine + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.297 heterotrimer subunits Lac1p, Lag1p, and Lip1p Saccharomyces cerevisiae
2.3.1.297 More Lag1p, Lac1p and Lip1p comprise the ceramide synthase. Lag1p/Lac1p interacting protein, 28 kDa, Lip1p is an essential component of the ceramide synthase, which forms a heteromeric complex with Lac1p and Lag1p. Lip1p is required for ceramide synthesis in vivo and in vitro. It is possible that Lip1p is modified by O-linked glycosylation. Its short, highly charged N-terminus is cytoplasmic and dispensible for ceramide synthase activity Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
2.3.1.297 acyl-CoA ceramide synthase
-
 Saccharomyces cerevisiae
2.3.1.297 ceramide synthase complex
-
 Saccharomyces cerevisiae
2.3.1.297 Lac1p/Lag1p
-
 Saccharomyces cerevisiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.297 24
-
 assay at Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.297 6.8
-
 assay at Saccharomyces cerevisiae

General Information

EC Number General Information Comment Organism
2.3.1.297 physiological function Lag1p, Lac1p and Lip1p comprise the ceramide synthase. Lag1p and Lac1p are subunits of the acyl-CoA-dependent ceramide synthase. The other essential component of the ceramide synthase, Lip1p, which forms a heteromeric complex with Lac1p and Lag1p. Lip1p is required for ceramide synthesis in vivo and in vitro Saccharomyces cerevisiae