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Literature summary extracted from

  • Nilojan, J.; Bathige, S.D.N.K.; Kugapreethan, R.; Yang, H.; Kim, M.J.; Nam, B.H.; Lee, J.
    Molecular features and the transcriptional and functional delineation of complement system activators C1r and C1s from Sebastes schlegelii (2018), Dev. Comp. Immunol., 81, 279-290 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.41 expressed in Escherichia coli BL21 cells Sebastes schlegelii
3.4.21.42 expressed in Escherichia coli BL21 cells Sebastes schlegelii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.41 complement component C1s + H2O Sebastes schlegelii
-
 activated complement component C1s + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.41 Sebastes schlegelii A0A2I6BQJ9 black rockfish
-
3.4.21.42 Sebastes schlegelii A0A2I6BQK3 black rockfish
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.41 maltose affinity column chromatography Sebastes schlegelii
3.4.21.42 maltose affinity column chromatography Sebastes schlegelii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.41 gill high expression Sebastes schlegelii
-
3.4.21.41 liver highest expression Sebastes schlegelii
-
3.4.21.42 gill high expression Sebastes schlegelii
-
3.4.21.42 liver highest expression Sebastes schlegelii
-
3.4.21.42 spleen high expression Sebastes schlegelii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.41 complement component C1s + H2O
-
 Sebastes schlegelii activated complement component C1s + ?
-
 ?
3.4.21.41 succinylated casein + H2O
-
 Sebastes schlegelii ?
-
 ?
3.4.21.42 succinylated casein + H2O
-
 Sebastes schlegelii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.41 ? x * 69500, enzyme fused to maltose binding protein, SDS-PAGE Sebastes schlegelii
3.4.21.41 ? x * 75900, calculated from amino acid sequence Sebastes schlegelii
3.4.21.42 ? x * 74700, calculated from amino acid sequence Sebastes schlegelii
3.4.21.42 ? x * 62800, enzyme fused to maltose binding protein, SDS-PAGE Sebastes schlegelii

Synonyms

EC Number Synonyms Comment Organism
3.4.21.41 C1r
-
 Sebastes schlegelii
3.4.21.42 C1s
-
 Sebastes schlegelii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.41 Sebastes schlegelii calculated from amino acid sequence
-
 5.1
3.4.21.42 Sebastes schlegelii calculated from amino acid sequence
-
 5.2

Expression

EC Number Organism Comment Expression
3.4.21.41 Sebastes schlegelii the enzyme mRNA expression is significantly upregulated in spleen and liver after pathologic stress with Streptococcus iniae up
3.4.21.42 Sebastes schlegelii the enzyme mRNA expression is significantly upregulated in spleen and liver after pathologic stress with Streptococcus iniae up