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Literature summary extracted from

  • Xiang, J.; Li, X.; Chen, Y.; Lu, Y.; Yu, M.; Chen, X.; Zhang, W.; Zeng, Y.; Sun, L.; Chen, S.; Sha, Z.
    Complement factor I from flatfish half-smooth tongue (Cynoglossus semilaevis) exhibited anti-microbial activities (2015), Dev. Comp. Immunol., 53, 199-209 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.45 expressed in Escherichia coli BL21(DE3) cells Cynoglossus semilaevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.45 complement component C3b + H2O Cynoglossus semilaevis
-
 ?
-
 ?
3.4.21.45 complement component C4b + H2O Cynoglossus semilaevis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.45 Cynoglossus semilaevis A0A0H4CX09
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.45 His Trap column chromatography Cynoglossus semilaevis

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.21.45 dialysis with from 6 to 0 M urea Cynoglossus semilaevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.45 blood
-
 Cynoglossus semilaevis
-
3.4.21.45 brain lowest expression Cynoglossus semilaevis
-
3.4.21.45 gill
-
 Cynoglossus semilaevis
-
3.4.21.45 head kidney
-
 Cynoglossus semilaevis
-
3.4.21.45 heart
-
 Cynoglossus semilaevis
-
3.4.21.45 intestine high expression Cynoglossus semilaevis
-
3.4.21.45 liver predominant expression Cynoglossus semilaevis
-
3.4.21.45 muscle
-
 Cynoglossus semilaevis
-
3.4.21.45 ovary high expression Cynoglossus semilaevis
-
3.4.21.45 skin
-
 Cynoglossus semilaevis
-
3.4.21.45 spleen
-
 Cynoglossus semilaevis
-
3.4.21.45 stomach lowest expression Cynoglossus semilaevis
-
3.4.21.45 trunk kidney lowest expression Cynoglossus semilaevis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.45 complement component C3b + H2O
-
 Cynoglossus semilaevis ?
-
 ?
3.4.21.45 complement component C4b + H2O
-
 Cynoglossus semilaevis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.45 ? x * 69230, mature protein, calculated from amino acid sequence Cynoglossus semilaevis
3.4.21.45 ? x * 71000, His6-tagged enzyme, SDS-PAGE Cynoglossus semilaevis
3.4.21.45 ? x * 72280, calculated from amino acid sequence Cynoglossus semilaevis

Synonyms

EC Number Synonyms Comment Organism
3.4.21.45 CFI
-
 Cynoglossus semilaevis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.45 Cynoglossus semilaevis calculated from amino acid sequence
-
 7.1

Expression

EC Number Organism Comment Expression
3.4.21.45 Cynoglossus semilaevis enzyme expression is increased after challenge with Vibrio anguillarum up

General Information

EC Number General Information Comment Organism
3.4.21.45 physiological function the enzyme plays an important role in Cynoglossus semilaevis immunity and shows broad-spectrum antimicrobial activities against the Gram-positive bacteria Staphylococcus aureus and the Gram-negative bacteria Escherichia coli, Pseudomonas aeruginosa and Shewanella putrefaciens Cynoglossus semilaevis