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Literature summary extracted from
- Alterations of histone modifications by cobalt compounds (2009), Carcinogenesis, 30, 1243-1251 .
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.66 | ascorbate | required | Homo sapiens |  |
| 1.14.11.69 | ascorbate | required | Homo sapiens | |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.66 | gene KDM4A, recombinant expression of FLAG-tagged JMJD2A in Spodoptera frugiperda Sf9 cells | Homo sapiens |
| 1.14.11.69 | gene KDM4A, recombinant expression of FLAG-tagged JMJD2A in Spodoptera frugiperda Sf9 cells | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.66 | Co2+ | has an activating on multiple histone modifications at the global level. Cobalt ions significantly increase global histone H3K4me3, H3K9me2, H3K9me3, H3K27me3 and H3K36me3, as well as uH2A and uH2B and decreases acetylation at histone H4 (AcH4) in vivo. Cobalt ions increase H3K9me3 and H3K36me3 by inhibiting histone demethylation process in vivo. And cobalt ions directly inhibit demethylase activity of JMJD2A in vitro. Cobalt ions do not increase the level of uH2A in the in vitro histone ubiquitinating assay and inhibit histone-deubiquitinating enzyme activity in vitro | Homo sapiens | |
| 1.14.11.69 | Co2+ | has an activating on multiple histone modifications at the global level. Cobalt ions significantly increase global histone H3K4me3, H3K9me2, H3K9me3, H3K27me3 and H3K36me3, as well as uH2A and uH2B and decreases acetylation at histone H4 (AcH4) in vivo. Cobalt ions increase H3K9me3 and H3K36me3 by inhibiting histone demethylation process in vivo. And cobalt ions directly inhibit demethylase activity of JMJD2A in vitro. Cobalt ions do not increase the level of uH2A in the in vitro histone ubiquitinating assay and inhibit histone-deubiquitinating enzyme activity in vitro | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.11.66 | nucleus | - |
Homo sapiens | 5634 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.66 | Fe2+ | required for activity | Homo sapiens | |
| 1.14.11.69 | Fe2+ | required for activity | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.66 | [histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.66 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.69 | [histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.69 | [histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2 | Homo sapiens | - |
[histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.66 | Homo sapiens | O75164 | - |
- |
| 1.14.11.69 | Homo sapiens | O75164 | - |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.66 | A-549 cell | - |
Homo sapiens | - |
| 1.14.11.66 | BEAS-2B cell | - |
Homo sapiens | - |
| 1.14.11.66 | bronchial epithelial cell | - |
Homo sapiens | - |
| 1.14.11.66 | lung cancer cell | - |
Homo sapiens | - |
| 1.14.11.69 | A-549 cell | - |
Homo sapiens | - |
| 1.14.11.69 | BEAS-2B cell | - |
Homo sapiens | - |
| 1.14.11.69 | bronchial epithelial cell | - |
Homo sapiens | - |
| 1.14.11.69 | lung cancer cell | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.66 | additional information | JMJD2A also catalyzes the reaction of the [histone H3]-lysine-9 demethylase. JMJD2A exclusively catalyzes the demethylation of H3K9me3 and H3K36me3, converting H3K9/36me3 to H3K9/36me2 but it cannot convert H3K9/36me1 or unmethylated H3K9/K36, overview | Homo sapiens | ? | - |
? | |
| 1.14.11.66 | [histone H3]-N6,N6,N6-trimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6,N6-dimethyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.66 | [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.69 | additional information | JMJD2A also catalyzes the reaction of the [histone H3]-lysine-9 demethylase. JMJD2A exclusively catalyzes the demethylation of H3K9me3 and H3K36me3, converting H3K9/36me3 to H3K9/36me2 but it cannot convert H3K9/36me1 or unmethylated H3K9/K36, overview | Homo sapiens | ? | - |
? | |
| 1.14.11.69 | [histone H3]-N6,N6,N6-trimethyl-L-lysine 36 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6,N6-dimethyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
| 1.14.11.69 | [histone H3]-N6,N6-dimethyl-L-lysine 36 + 2-oxoglutarate + O2 | - |
Homo sapiens | [histone H3]-N6-methyl-L-lysine 36 + succinate + formaldehyde + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.66 | histone H3K9/H3K36 trimethyldemethylase | - |
Homo sapiens |
| 1.14.11.66 | JMJD2A | - |
Homo sapiens |
| 1.14.11.66 | JMJD2A demethylase | - |
Homo sapiens |
| 1.14.11.66 | KDM4A | - |
Homo sapiens |
| 1.14.11.66 | More | see also EC 1.14.11.69 | Homo sapiens |
| 1.14.11.66 | [histone H3]-lysine-9 demethylase | - |
Homo sapiens |
| 1.14.11.69 | histone H3K9/H3K36 trimethyldemethylase | - |
Homo sapiens |
| 1.14.11.69 | JMJD2A | - |
Homo sapiens |
| 1.14.11.69 | JMJD2A demethylase | - |
Homo sapiens |
| 1.14.11.69 | KDM4A | - |
Homo sapiens |
| 1.14.11.69 | lysine trimethyl-specific JmjC histone demethylase | - |
Homo sapiens |
| 1.14.11.69 | More | see also EC 1.14.11.66 | Homo sapiens |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.66 | 37 | - |
assay at | Homo sapiens |
| 1.14.11.69 | 37 | - |
assay at | Homo sapiens |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.66 | 8 | - |
assay at | Homo sapiens |
| 1.14.11.69 | 8 | - |
assay at | Homo sapiens |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.14.11.66 | metabolism | exposure to Co2+ increases gene repression markers (H3K9me3, H3K27me3, H3K36me3, H3K9me2, uH2A and lack of AcH4), as well as gene activation markers (H3K4me3 and uH2B) in both A549 and Beas-2B cells. Cobalt ions increase H3K9me3 and H3K36me3 by inhibiting histone demethylation process in vivo | Homo sapiens |
| 1.14.11.69 | metabolism | exposure to Co2+ increases gene repression markers (H3K9me3, H3K27me3, H3K36me3, H3K9me2, uH2A and lack of AcH4), as well as gene activation markers (H3K4me3 and uH2B) in both A549 and Beas-2B cells. Cobalt ions increase H3K9me3 and H3K36me3 by inhibiting histone demethylation process in vivo | Homo sapiens |
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