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Literature summary extracted from

  • Ivanov, I.; Matafonov, A.; Sun, M.F.; Cheng, Q.; Dickeson, S.K.; Verhamme, I.M.; Emsley, J.; Gailani, D.
    Proteolytic properties of single-chain factor XII a mechanism for triggering contact activation (2017), Blood, 129, 1527-1537 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.21.38 alpha-kallikrein
-
 Homo sapiens
3.4.21.38 additional information the enzyme gets activated by contact when exposed to artificial surfaces and biological substances Homo sapiens
3.4.21.38 Polyphosphate
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.4.21.38 R334A/R343A/R353A the mutant lacks the Arg334, Arg343, and Arg353 cleavage sites required for conversion to the enzyme form betaFXIIa and shows proteolytic activity that is about 3000fold weaker than that of the wild type enzyme Homo sapiens
3.4.21.38 R353A the mutant lacks the Arg353 cleavage site required for conversion to the activated enzyme form alphaFXIIa and shows proteolytic activity that is several orders of magnitude weaker than that of the wild type enzyme Homo sapiens
3.4.21.38 S544A active site mutant Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.38 0.0008
-
 prekallikrein mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 0.0017
-
 prekallikrein wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 0.1
-
 S-2302 wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 2.7
-
 S-2302 mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.38 factor XI + H2O Homo sapiens
-
 factor XIa + ?
-
 ?
3.4.21.38 prekallikrein + H2O Homo sapiens
-
 kallikrein + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.38 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.38 blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.38 factor XI + H2O
-
 Homo sapiens factor XIa + ?
-
 ?
3.4.21.38 prekallikrein + H2O
-
 Homo sapiens kallikrein + ?
-
 ?
3.4.21.38 S-2302 + H2O
-
 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.38 ? x * 80000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.38 alphaFXIIa
-
 Homo sapiens
3.4.21.38 single-chain FXII
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.38 0.000062
-
 prekallikrein mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 0.108
-
 S-2302 mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 0.73
-
 prekallikrein wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 11.7
-
 S-2302 wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.4.21.38 0.077
-
 prekallikrein mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 0.4
-
 S-2302 mutant enzyme R334A/R343A/R353A, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 116.7
-
 S-2302 wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens
3.4.21.38 4333
-
 prekallikrein wild type enzyme alphaFXIIa, at pH 7.4 and 37°C Homo sapiens