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Literature summary extracted from

  • Kim, H.G.; Kim, S.W.
    Purification and characterization of a methanol dehydrogenase derived from Methylomicrobium sp. HG-1 cultivated using a compulsory circulation diffusion system (2006), Biotechnol. Bioprocess Eng., 11, 134-139 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.2.7 Co2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
1.1.2.7 Cu2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
1.1.2.7 Fe2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
1.1.2.7 Mn2+ 1 mM complete inhibition Methylomicrobium sp. HG-1
1.1.2.7 additional information 1 mM of Zn2+, Mg2+, and Ca2+ cause complete inhibition, EDTA inhibits in vivo Methylomicrobium sp. HG-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.2.7 0.81
-
 methanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
1.1.2.7 5.73
-
 ethanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
1.1.2.7 80.78
-
 Heptanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
1.1.2.7 162
-
 Hexanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
1.1.2.7 172
-
 Pentanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
1.1.2.7 184
-
 Propanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1
1.1.2.7 186
-
 butanol 100 mM Tris-HCl buffer (pH 9.0), 1.1 mM phenazine ethosulfate (initial electron acceptor), 0.04 mM 2,6-dichlorophenol indophenol (terminal electron acceptor), 1 mM KCN and 15 mM NH4Cl, 60°C Methylomicrobium sp. HG-1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.2.7 cytoplasm
-
 Methylomicrobium sp. HG-1 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.2.7 methanol + 2 oxidized cytochrome cL Methylomicrobium sp. HG-1
-
 formaldehyde + 2 reduced cytochrome cL
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.2.7 Methylomicrobium sp. HG-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.2.7 soluble fraction concentrated with Centricon (Millipore, Billerica, Mass, USA), applied to a POROS 20 HQ column, followed by FPLC Superose 12 HR 10/30 column Methylomicrobium sp. HG-1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.2.7 4210
-
 reduction of 4210 micromol 2,6-dichlorophenol indophenol per min, pH 9.0, 30°C Methylomicrobium sp. HG-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.2.7 acetaldehyde + 2 reduced cytochrome cL 7.06% of the activity with methanol Methylomicrobium sp. HG-1 ethanol + 2 oxidizeded cytochrome cL
-
 ?
1.1.2.7 butanol + 2 oxidized cytochrome cL 49.13% of the activity with methanol Methylomicrobium sp. HG-1 butanal + 2 reduced cytochrome cL
-
 ?
1.1.2.7 ethanol + 2 oxidized cytochrome cL 85.13% of the activity with methanol Methylomicrobium sp. HG-1 acetaldehyde + 2 reduced cytochrome cL
-
 ?
1.1.2.7 formaldehyde + 2 reduced cytochrome cL 73.24% of the activity with methanol Methylomicrobium sp. HG-1 methanol + 2 oxidizeded cytochrome cL
-
 ?
1.1.2.7 heptanol + 2 oxidized cytochrome cL 101.88% of the activity with methanol Methylomicrobium sp. HG-1 heptanal + 2 reduced cytochrome cL
-
 ?
1.1.2.7 hexanol + 2 oxidized cytochrome cL 94.10% of the activity with methanol Methylomicrobium sp. HG-1 hexanal + 2 reduced cytochrome cL
-
 ?
1.1.2.7 methanol + 2 oxidized cytochrome cL
-
 Methylomicrobium sp. HG-1 formaldehyde + 2 reduced cytochrome cL
-
 ?
1.1.2.7 octanol + 2 oxidized cytochrome cL 16.33% of the activity with methanol Methylomicrobium sp. HG-1 octanal + 2 reduced cytochrome cL
-
 ?
1.1.2.7 pentanol + 2 oxidized cytochrome cL 67.40% of the activity with methanol Methylomicrobium sp. HG-1 pentanal + 2 reduced cytochrome cL
-
 ?
1.1.2.7 propanol + 2 oxidized cytochrome cL 59.93% of the activity with methanol Methylomicrobium sp. HG-1 propionaldehyde + 2 reduced cytochrome cL
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.2.7 60
-
-
 Methylomicrobium sp. HG-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.2.7 9
-
-
 Methylomicrobium sp. HG-1

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.2.7 cytochrome cL
-
 Methylomicrobium sp. HG-1