Any feedback?
Literature summary extracted from
- Structure and function of legumain in health and disease (2016), Biochimie, 122, 126-150 .
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.4.22.34 | Cu2+ | - |
Bos taurus |  |
| 3.4.22.34 | Hg2+ | - |
Bos taurus | |
| 3.4.22.34 | additional information | the enzyme is not inhibited by the broad spectrum cysteine protease inhibitors E-64, PMSF nor by the aspartate protease inhibitor pepstatin A | Bos taurus | |
| 3.4.22.34 | additional information | the enzyme is not inhibited by the broad spectrum cysteine protease inhibitors E-64, PMSF nor by the aspartate protease inhibitor pepstatin A | Homo sapiens | |
| 3.4.22.34 | additional information | the enzyme is not inhibited by the broad spectrum cysteine protease inhibitors E-64, PMSF nor by the aspartate protease inhibitor pepstatin A | Mammalia | |
| 3.4.22.34 | additional information | the enzyme is not inhibited by the broad spectrum cysteine protease inhibitors E-64, PMSF nor by the aspartate protease inhibitor pepstatin A | Schistosoma mansoni | |
| 3.4.22.34 | additional information | the enzyme is not inhibited by the broad spectrum cysteine protease inhibitors E-64, PMSF nor by the aspartate protease inhibitor pepstatin A | unidentified plant |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.4.22.34 | cytosol | - |
Mammalia | 5829 | - |
| 3.4.22.34 | extracellular | - |
Mammalia | - |
- |
| 3.4.22.34 | lysosome | - |
Mammalia | 5764 | - |
| 3.4.22.34 | nucleus | - |
Mammalia | 5634 | - |
| 3.4.22.34 | vacuole | - |
unidentified plant | 5773 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | Bos taurus | Q95M12 | - |
- |
| 3.4.22.34 | Homo sapiens | Q99538 | - |
- |
| 3.4.22.34 | Mammalia | - |
- |
- |
| 3.4.22.34 | Schistosoma mansoni | P09841 | - |
- |
| 3.4.22.34 | unidentified plant | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | glycoprotein | the enzyme harbours 4 N-glycosylation sites resulting in a molecular weight of about 56000 Da of the fully glycosylated proenzyme | Homo sapiens |
| 3.4.22.34 | proteolytic modification | human prolegumain starts with a signal peptide (Met1-Ala17) that is released during trafficking, followed by a short 8 amino acid N-terminal propeptide (Val18-Asp25), the cysteine protease domain (Gly26-Asn323) and a C-terminal prodomain (Asp324-Tyr433) | Homo sapiens |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.22.34 | breast cancer cell | legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer | Homo sapiens | - |
| 3.4.22.34 | colorectal cancer cell | legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer | Homo sapiens | - |
| 3.4.22.34 | gastric cancer cell | legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer | Homo sapiens | - |
| 3.4.22.34 | ovary cancer cell | legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer | Homo sapiens | - |
| 3.4.22.34 | pericarp | - |
unidentified plant | - |
| 3.4.22.34 | prostate gland cancer cell | legumain is overexpressed in the majority of human solid tumours, including breast cancer, colorectal cancer, ovarian cancer, prostate cancer and gastric cancer | Homo sapiens | - |
| 3.4.22.34 | seed | - |
unidentified plant | - |
| 3.4.22.34 | vegetative cell | - |
unidentified plant | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.22.34 | 11S/12S proglobulin + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | acetoacetyl-CoA synthetase + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | Albumin + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | annexin A2 + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | Bovine serum albumin + H2O | - |
Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | cathepsin B + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | cathepsin H + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | cathepsin L + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | cathepsin S + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | cyclic knottin + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | cystatin C + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | cystatin E + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | Fibronectin + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | Gliadin + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | globulin + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | glutelin + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | Hemoglobin + H2O | - |
Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | Kalata-type cyclic peptide + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | additional information | contrasting its endopeptidase activity, legumain can develop a carboxypeptidase activity which remains stable at neutral pH | Mammalia | ? | - |
? | |
| 3.4.22.34 | additional information | ideal cleavage sequence: Pro-Thr-Asn (P3-P2-P1) | Homo sapiens | ? | - |
? | |
| 3.4.22.34 | additional information | ideal cleavage sequence: Thr-Ala-Asn (P3-P2-P1) | Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | additional information | plant legumain shows a preference for bulky residues at P3 position | unidentified plant | ? | - |
? | |
| 3.4.22.34 | myelin basic protein + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | proconcanavalin A + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | progelatinase A + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | prolegumain + H2O | - |
Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | prolegumain + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | Schistosoma cathepsin B + H2O | - |
Schistosoma mansoni | ? | - |
? | |
| 3.4.22.34 | serine protease inhibitor precursor + H2O | - |
unidentified plant | ? | - |
? | |
| 3.4.22.34 | Tau-protein + H2O | - |
Mammalia | ? | - |
? | |
| 3.4.22.34 | Tetanus toxin C-terminal fragment + H2O | - |
Mammalia | ? | - |
? | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 3.4.22.34 | malfunction | in pathological settings, such as cancer or Alzheimer's disease, the proper association of legumain activities with the corresponding cellular compartments is breached | Mammalia |
| 3.4.22.34 | physiological function | legumain promotes cell migration and its overexpression is associated with enhanced tissue invasion and metastasis. It promotes tumorigenesis and correlates with poor prognosis in different cancer types | Homo sapiens |
| 3.4.22.34 | physiological function | plant legumains are key enzymes in the processing and activation of seed storage proteins during seed maturation. Plants legumains are important components of the immune system. Plant legumains are discussed as main mediators of programmed cell death | unidentified plant |
| 3.4.22.34 | physiological function | the lysosomal enzyme contributes to antigen processing for class II MHC presentation | Mammalia |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
