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Literature summary extracted from

  • Gaweska, H.; Henderson Pozzi, M.; Schmidt, D.M.; McCafferty, D.G.; Fitzpatrick, P.F.
    Use of pH and kinetic isotope effects to establish chemistry as rate-limiting in oxidation of a peptide substrate by LSD1 (2009), Biochemistry, 48, 5440-5445 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.99.66 additional information
-
 additional information Michaelis-Menten steady-state kinetics, stopped-flow data, kinetic isotope effects at different pH values from pH 7.0-9.5, overview Homo sapiens
1.14.99.66 0.0026
-
 H3K4me2 (1-21 aa) peptide recombinant enzyme LSD1, pH 7.5, 25°C Homo sapiens
1.14.99.66 0.195
-
 O2 recombinant enzyme LSD1, pH 7.5, 25°C, with H3K4me2 (1-21 aa) peptide Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.11.65 nucleus
-
 Homo sapiens 5634
-
1.14.99.66 nucleus
-
 Homo sapiens 5634
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.11.65 [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
1.14.99.66 [histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?
1.14.99.66 [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2 Homo sapiens
-
 [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.65 Homo sapiens O60341
-
-
1.14.99.66 Homo sapiens O60341
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.11.65 additional information the bifunctional enzyme catalyzes the demethylation of H3K4me2/me1 (EC 1.14.99.66) and H3K9me2/me1 Homo sapiens ?
-
 ?
1.14.11.65 [histone H3]-N6,N6-dimethyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
1.14.11.65 [histone H3]-N6-methyl-L-lysine 9 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-L-lysine 9 + succinate + formaldehyde + CO2
-
 ?
1.14.99.66 H3K4me2 (1-21 aa) peptide + 2-oxoglutarate + O2 a peptide corresponding to the first 21 amino acids of histone H3 with a dimethylated lysine at the fourth residue [ARTK(diMe)QTARKSTGGKAPRKQLA] Homo sapiens H3K4me1 (1-21 aa) peptide + succinate + formaldehyde + CO2
-
 ?
1.14.99.66 additional information the bifunctional enzyme catalyzes the demethylation of H3K4me2/me1 and H3K9me2/me1 (EC 1.14.11.65) Homo sapiens ?
-
 ?
1.14.99.66 [histone H3]-N6,N6-dimethyl-L-lysine 4 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-N6-methyl-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?
1.14.99.66 [histone H3]-N6-methyl-L-lysine 4 + 2-oxoglutarate + O2
-
 Homo sapiens [histone H3]-L-lysine 4 + succinate + formaldehyde + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.11.65 LSD1
-
 Homo sapiens
1.14.11.65 lysine-specific demethylase 1
-
 Homo sapiens
1.14.11.65 More see also EC 1.14.99.66 Homo sapiens
1.14.99.66 LSD1
-
 Homo sapiens
1.14.99.66 lysine-specific demethylase 1
-
 Homo sapiens
1.14.99.66 More see also EC 1.14.11.65 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.11.65 25
-
 assay at Homo sapiens
1.14.99.66 25
-
 assay at Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.99.66 0.199
-
 H3K4me2 (1-21 aa) peptide recombinant enzyme LSD1, pH 7.5, 25°C Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.11.65 7.5
-
 assay at Homo sapiens
1.14.99.66 7.5
-
 assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.11.65 FAD required Homo sapiens
1.14.99.66 FAD dependent on Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.99.66 76.5
-
 H3K4me2 (1-21 aa) peptide recombinant enzyme LSD1, pH 7.5, 25°C Homo sapiens