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Literature summary extracted from

  • Lo, S.Y.; Saebel, C.E.; Mapletoft, J.P.J.; Siemann, S.
    Influence of chemical denaturants on the activity, fold and zinc status of anthrax lethal factor (2015), Biochem. Biophys. Rep., 1, 68-77 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.24.83 expressed in Bacillus megaterium Bacillus anthracis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.83 guanidine hydrochloride
-
 Bacillus anthracis
3.4.24.83 guanidine isothiocyanate
-
 Bacillus anthracis
3.4.24.83 MgSO4
-
 Bacillus anthracis
3.4.24.83 Na2SO4
-
 Bacillus anthracis
3.4.24.83 NaCl
-
 Bacillus anthracis
3.4.24.83 NaSCN
-
 Bacillus anthracis
3.4.24.83 Urea
-
 Bacillus anthracis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.83 Zn2+ contains zinc Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.83 mitogen-activated protein kinase kinase 1 + H2O Bacillus anthracis
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.83 Bacillus anthracis P15917
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.83 acetyl-GYbetaARRRRRRRRVLR-4-nitroanilide + H2O
-
 Bacillus anthracis ?
-
 ?
3.4.24.83 mitogen-activated protein kinase kinase 1 + H2O
-
 Bacillus anthracis ?
-
 ?

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.83 2 5 MgSO4 at pH 7.4 and 25°C Bacillus anthracis
3.4.24.83 50
-
 Na2SO4 at pH 7.4 and 25°C Bacillus anthracis
3.4.24.83 140
-
 guanidine hydrochloride at pH 7.4 and 25°C Bacillus anthracis
3.4.24.83 140
-
 guanidine isothiocyanate at pH 7.4 and 25°C Bacillus anthracis
3.4.24.83 150
-
 NaCl at pH 7.4 and 25°C Bacillus anthracis
3.4.24.83 150
-
 NaSCN at pH 7.4 and 25°C Bacillus anthracis
3.4.24.83 1050
-
 Urea at pH 7.4 and 25°C Bacillus anthracis