EC Number | General Stability | Organism |
---|---|---|
3.5.1.14 | Zn2+, 1 mM or 10 mM, enhances thermostability | Geobacillus sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.14 | additional information | treatment of the enzyme with EDTA at 5 and 10 mM does not inhibit the activity | Geobacillus sp. | |
3.5.1.14 | PMSF | 10 mM, relative activity decreases to 91.6% of control | Geobacillus sp. | |
3.5.1.14 | SDS | 10 mM, relative activity decreases to 85.4% of control | Geobacillus sp. | |
3.5.1.14 | Triton X-100 | 5 mM and 10 mM, relative activity is decreases from 73% to 66.4% | Geobacillus sp. | |
3.5.1.14 | Tween 20 | 5 mM and 10 mM, relative activity is decreases from 90.7% to 85.8% | Geobacillus sp. | |
3.5.1.14 | Zn2+ | 1 mM or 10 mM | Geobacillus sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.14 | Cu2+ | 1 mM, increases aminoacylase activity to 107% | Geobacillus sp. | |
3.5.1.14 | Mg2+ | 1 mM, increases aminoacylase activity to 153% | Geobacillus sp. | |
3.5.1.14 | Mn2+ | 1 mM, increases aminoacylase activity to 160% | Geobacillus sp. | |
3.5.1.14 | Na+ | 1 mM, increases aminoacylase activity to 136% | Geobacillus sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 42000 | - |
SDS-PAGE | Geobacillus sp. |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.14 | Geobacillus sp. | - |
- |
- |
3.5.1.14 | Geobacillus sp. SZN | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.14 | - |
Geobacillus sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 66.4 | - |
pH 7.5, 60°C | Geobacillus sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.14 | N-acetyl-L-methionine + H2O | - |
Geobacillus sp. | acetic acid + L-methionine | - |
? | |
3.5.1.14 | N-acetyl-L-methionine + H2O | - |
Geobacillus sp. SZN | acetic acid + L-methionine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.5.1.14 | ? | x * 42000, SDS-PAGE | Geobacillus sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 60 | - |
- |
Geobacillus sp. |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 30 | 90 | 30°C: about 37.6% of maximal activity, 90°C: about 27% of maximal activity | Geobacillus sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | additional information | - |
thermostable enzyme based on the alpha-helical structure integrity and functional stability in high temperatures | Geobacillus sp. |
3.5.1.14 | 60 | - |
20 h, 53% loss of activity. 32 h, 72% loss of activity | Geobacillus sp. |
3.5.1.14 | 70 | - |
the alpha-helix, where notable metal binding and catalytic residues are located, is totally weakened at temperatures above 70°C and thus results in loss of activity. The loss of the alpha-helical structure can explain drastic deterioration of activity at temperatures beyond 70°C | Geobacillus sp. |
3.5.1.14 | 80 | - |
thermal denaturation point | Geobacillus sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 8 | - |
- |
Geobacillus sp. |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.14 | 4 | 11 | pH 4.0: 59% of maximal activity, pH 11.0: 19% of maximal activity | Geobacillus sp. |