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Literature summary extracted from

  • Shibata, M.; Koike, M.; Kusumi, S.; Sato, N.; Uchiyama, Y.
    A specific tripeptidyl substrate for tripeptidyl peptidase activity is effectively hydrolyzed by alanyl aminopeptidase/aminopeptidase N/CD13 in the rat kidney (2016), Arch. Histol. Cytol., 76, 1-8 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 1,10-phenanthroline complete inhibition at 1 mM Rattus norvegicus
3.4.11.2 amastatin 0.1 mM, 1% residual activity; 99% inhibiton at 0.1 mM Rattus norvegicus
3.4.11.2 bestatin 0.1 mM, 7% residual activity; 93% inhibiton at 0.1 mM Rattus norvegicus
3.4.11.2 Co2+ 58% inhibition at 1 mM Rattus norvegicus
3.4.11.2 copper 1 mM, 5% resiudal activity Rattus norvegicus
3.4.11.2 Cu2+ 95% inhibition at 1 mM Rattus norvegicus
3.4.11.2 EDTA 23% inhibition at 5 mM; 5 mM, 77% residual activity Rattus norvegicus
3.4.11.2 EGTA 15% inhibition at 5 mM; 5 mM, 85% residual activity Rattus norvegicus
3.4.11.2 Mn2+ 44% inhibition at 1 mM Rattus norvegicus
3.4.11.2 Ni2+ 71% inhibition at 1 mM Rattus norvegicus
3.4.11.2 o-phenanthroline 1 mM, no residual activity Rattus norvegicus
3.4.11.2 Zinc 1 mM, 1% resiudal activity Rattus norvegicus
3.4.11.2 Zn2+ 99% inhibition at 1 mM Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 brush border
-
 Rattus norvegicus 5903
-
3.4.11.2 soluble
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 additional information no or poor effects by 1 mM of Mg2+ and Ca2+ Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Rattus norvegicus P15684
-
-
3.4.11.2 Rattus norvegicus Wistar P15684
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.2
-
 Rattus norvegicus
3.4.11.2 native enzyme partially from rat kidney by gel filtration Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 bile canaliculus surface membrane Rattus norvegicus
-
3.4.11.2 hepatocyte positive staining for the enzyme is limited strictly to the membrane of the hepatocytes that consist of bile canaliculi Rattus norvegicus
-
3.4.11.2 kidney located in brush border of a renal proximal tubule and the small intestine, and the surface membrane of bile canaliculi Rattus norvegicus
-
3.4.11.2 kidney renal proximal tubule, no immunoreactivity to the antiserum is found in the glomeruli, in the distal renal tubular epithelial cells, or in the renal tubules in the medulla Rattus norvegicus
-
3.4.11.2 liver located in brush border of a renal proximal tubule and the small intestine, and the surface membrane of bile canaliculi Rattus norvegicus
-
3.4.11.2 liver positive staining for the enzyme is limited strictly to the membrane of the hepatocytes that consist of bile canaliculi, but no signal is seen in the sinusoidal wall. The low expression of the enzyme in the liver might be due to the limited localization on the bile canaliculi Rattus norvegicus
-
3.4.11.2 additional information immunohistochemic analysis Rattus norvegicus
-
3.4.11.2 renal proximal tubule
-
 Rattus norvegicus
-
3.4.11.2 small intestine brush border of the epithelium in the small intestine Rattus norvegicus
-
3.4.11.2 small intestine located in brush border of a renal proximal tubule and the small intestine, and the surface membrane of bile canaliculi Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 L-Ala-7-amido-4-carbamoylmethylcoumarin + H2O
-
 Rattus norvegicus L-Ala + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-Ala-7-amido-4-carbamoylmethylcoumarin + H2O
-
 Rattus norvegicus Wistar L-Ala + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-alanine 4-methylcoumaryl-7-amide + H2O Ala-MCA Rattus norvegicus L-alanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-alanyl-L-alanyl-L-7-amido-4-carbamoylmethylcoumarin + H2O substrate for use with tripeptidyl peptidases Rattus norvegicus L-alanyl-L-alanine + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-alanyl-L-alanyl-L-phenylalanine 4-methylcoumaryl-7-amide + H2O Ala-Ala-Phe-MCA or AAF-MCA Rattus norvegicus L-alanyl-L-alanyl-L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-arginine 4-methylcoumaryl-7-amide + H2O Arg-MCA Rattus norvegicus L-arginine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Leu-7-amido-4-carbamoylmethylcoumarin + H2O 70% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin Rattus norvegicus L-Leu + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-Leu-7-amido-4-carbamoylmethylcoumarin + H2O 70% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin Rattus norvegicus Wistar L-Leu + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-leucine 4-methylcoumaryl-7-amide + H2O Leu-MCA Rattus norvegicus L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-lysine 4-methylcoumaryl-7-amide + H2O Lys-MCA, low activity Rattus norvegicus L-lysine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Met-7-amido-4-carbamoylmethylcoumarin + H2O 99% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin Rattus norvegicus L-Met + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-Met-7-amido-4-carbamoylmethylcoumarin + H2O 99% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin Rattus norvegicus Wistar L-Met + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-methionine 4-methylcoumaryl-7-amide + H2O Met-MCA Rattus norvegicus L-methionine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 L-Phe-7-amido-4-carbamoylmethylcoumarin + H2O 76% of the activity with L-Ala-7-amido-4-carbamoylmethylcoumarin Rattus norvegicus L-Phe + 7-amino-4-carbamoylmethylcoumarin
-
 ?
3.4.11.2 L-phenylalanine 4-methylcoumaryl-7-amide + H2O Phe-MCA Rattus norvegicus L-phenylalanine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.2 additional information enzyme preferentially cleaves neutral and nonpolar amino acids such as alanine, methionine, leucine, and phenylalanine at the P1 position, substrates with basic amino acids such as lysine and arginine at the P1 position are less cleaved. Pyroglutamic acid, and Bz-Arginine, are not cleaved at all Rattus norvegicus ?
-
 ?
3.4.11.2 additional information the peptidase that hydrolyzes AAF-MCA is the soluble form of aminopeptidase N/CD13, and caution is required when using AAF-MCA as a substrate for tripeptidyl peptidase assays. No activity with Pyr-MCA and Bz-Arg-MCA Rattus norvegicus ?
-
 ?
3.4.11.2 additional information enzyme preferentially cleaves neutral and nonpolar amino acids such as alanine, methionine, leucine, and phenylalanine at the P1 position, substrates with basic amino acids such as lysine and arginine at the P1 position are less cleaved. Pyroglutamic acid, and Bz-Arginine, are not cleaved at all Rattus norvegicus Wistar ?
-
 ?
3.4.11.2 additional information the peptidase that hydrolyzes AAF-MCA is the soluble form of aminopeptidase N/CD13, and caution is required when using AAF-MCA as a substrate for tripeptidyl peptidase assays. No activity with Pyr-MCA and Bz-Arg-MCA Rattus norvegicus Wistar ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 ? x * 137000, SDS-PAGE Rattus norvegicus
3.4.11.2 ? x * 137000, enzyme from kidney, SDS-PAGE, x * 110000, enzyme from small intestine, SDS-PAGE, x * 140000, enzyme from liver, SDS-PAGE Rattus norvegicus
3.4.11.2 More the N-terminal amino acid sequence of the peptidase is x-Ala-Pro-x-Leu-Pro-Gly-Ser-Thr-Ser-Ala-Thr-x-x-Ser, where x indicates undetectable amino acid residues. The undetectable amino acid residues such as threonine or serine might be modified residues that often undergo glycosylation or phosphorylation Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 alanyl aminopeptidase
-
 Rattus norvegicus
3.4.11.2 aminopeptidase N
-
 Rattus norvegicus
3.4.11.2 ANPEP
-
 Rattus norvegicus
3.4.11.2 CD13
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.2 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.2 7.5
-
 assay at Rattus norvegicus