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Literature summary extracted from

  • Lv, M.; Zhang, Y.J.; Zhou, F.; Ge, Y.; Zhao, M.H.; Liu, Y.; Yang, K.W.
    Real-time monitoring of D-Ala-D-Ala dipeptidase activity of VanX in living bacteria by isothermal titration calorimetry (2019), Anal. Biochem., 578, 29-35 .
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.13.22 analysis continuous assay of VanX in vitro and in vivo from hydrolysis of D-Ala-D-Ala, based on the heat-rate changes measured with isothermal titration calorimetry Enterococcus faecalis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.13.22 expression in Escherichia coli and clinical strain VRE Enterococcus faecalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.13.22 D-cysteine
-
 Enterococcus faecalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.13.22 0.1
-
 D-Ala-D-Ala pH 8.0, 37°C Enterococcus faecalis

Organism

EC Number Organism UniProt Comment Textmining
3.4.13.22 Enterococcus faecalis
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.13.22 D-Ala-D-Ala + H2O
-
 Enterococcus faecalis 2 D-Ala
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.13.22 VanX
-
 Enterococcus faecalis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.13.22 11.5
-
 D-Ala-D-Ala pH 8.0, 37°C Enterococcus faecalis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.13.22 0.0188
-
 D-cysteine pH 8.0, 37°C Enterococcus faecalis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.13.22 0.0298
-
 pH 8.0, 37°C Enterococcus faecalis D-cysteine