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Literature summary extracted from

  • Upadhyaya, J.; Yoon, M.S.; Kim, M.J.; Ryu, N.S.; Song, Y.E.; Kim, Y.H.; Kim, M.K.
    Purification and characterization of a novel ginsenoside Rc-hydrolyzing beta-glucosidase from Armillaria mellea mycelia (2016), AMB Express, 6, 112 .
    View publication on PubMedView publication on EuropePMC

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.193 compound Mc + H2O Armillaria mellea
-
 compound K + L-arabinofuranose
-
 ?
3.2.1.193 compound Mc + H2O Armillaria mellea KACC 50013
-
 compound K + L-arabinofuranose
-
 ?
3.2.1.193 compound Mc1 + H2O Armillaria mellea
-
 compound Mc + D-glucose
-
 ?
3.2.1.193 ginsenoside F2 + H2O Armillaria mellea
-
 compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Pleurotus ostreatus
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Ganoderma lucidum
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Armillaria mellea
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Ganoderma applanatum
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Sanghuangporus baumii
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Armillaria mellea
-
 compound Mc1 + D-glucose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Ganoderma lucidum KACC 42231
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Armillaria mellea KACC 50013
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Pleurotus ostreatus KACC 50356
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Ganoderma applanatum KACC 53688
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O Sanghuangporus baumii KACC 53719
-
 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rd + H2O Armillaria mellea
-
 ginsenoside F2 + D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.193 Armillaria mellea
-
-
-
3.2.1.193 Armillaria mellea KACC 50013
-
-
-
3.2.1.193 Ganoderma applanatum
-
-
-
3.2.1.193 Ganoderma applanatum KACC 53688
-
-
-
3.2.1.193 Ganoderma lucidum
-
-
-
3.2.1.193 Ganoderma lucidum KACC 42231
-
-
-
3.2.1.193 Pleurotus ostreatus
-
-
-
3.2.1.193 Pleurotus ostreatus KACC 50356
-
-
-
3.2.1.193 Sanghuangporus baumii
-
-
-
3.2.1.193 Sanghuangporus baumii KACC 53719
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.193 native enzyme 33.8fold from mycelium by ammonium sulfate fractionation, anion exchange chromatographydialysis, lyophilization, and gel filtration Armillaria mellea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.193 mycelium
-
 Pleurotus ostreatus
-
3.2.1.193 mycelium
-
 Ganoderma lucidum
-
3.2.1.193 mycelium
-
 Armillaria mellea
-
3.2.1.193 mycelium
-
 Ganoderma applanatum
-
3.2.1.193 mycelium
-
 Sanghuangporus baumii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.193 17.9
-
 substrate ginsenoside Rc, pH 4.5, 50°C Armillaria mellea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.193 4-nitrophenyl-alpha-L-arabinofuranoside + H2O 30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.193 4-nitrophenyl-alpha-L-arabinofuranoside + H2O 30% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea KACC 50013 4-nitrophenol + L-arabinofuranose
-
 ?
3.2.1.193 4-nitrophenyl-alpha-L-arabinopyranoside + H2O 10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea 4-nitrophenol + L-arabinopyranose
-
 ?
3.2.1.193 4-nitrophenyl-alpha-L-arabinopyranoside + H2O 10% activity compared to 4-nitrophenyl-beta-D-glucopyranoside Armillaria mellea KACC 50013 4-nitrophenol + L-arabinopyranose
-
 ?
3.2.1.193 4-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Armillaria mellea 4-nitrophenol + D-glucopyranose
-
 ?
3.2.1.193 compound Mc + H2O
-
 Armillaria mellea compound K + L-arabinofuranose
-
 ?
3.2.1.193 compound Mc + H2O
-
 Armillaria mellea KACC 50013 compound K + L-arabinofuranose
-
 ?
3.2.1.193 compound Mc1 + H2O
-
 Armillaria mellea compound Mc + D-glucose
-
 ?
3.2.1.193 ginsenoside F2 + H2O
-
 Armillaria mellea compound K + D-glucose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Pleurotus ostreatus ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Ganoderma lucidum ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Armillaria mellea ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Ganoderma applanatum ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Sanghuangporus baumii ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Ganoderma lucidum KACC 42231 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Armillaria mellea KACC 50013 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Pleurotus ostreatus KACC 50356 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Ganoderma applanatum KACC 53688 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Sanghuangporus baumii KACC 53719 ginsenoside Rd + L-arabinofuranose
-
 ?
3.2.1.193 ginsenoside Rc + H2O
-
 Armillaria mellea compound Mc1 + D-glucose
-
 ?
3.2.1.193 ginsenoside Rd + H2O
-
 Armillaria mellea ginsenoside F2 + D-glucose
-
 ?
3.2.1.193 additional information enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside Armillaria mellea ?
-
 ?
3.2.1.193 additional information the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma applanatum ?
-
 ?
3.2.1.193 additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Pleurotus ostreatus ?
-
 ?
3.2.1.193 additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma lucidum ?
-
 ?
3.2.1.193 additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Sanghuangporus baumii ?
-
 ?
3.2.1.193 additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma lucidum KACC 42231 ?
-
 ?
3.2.1.193 additional information enzyme BG-1 hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site or outer beta-(1->2)-glucosidic linkage at the C-3 site of G-Rc to produce ginsenoside Rd and compound Mc1, respectively. The enzyme also hydrolyzes outer and inner glucosidic linkages at the C-3 site of ginsenoside Rd to produce compound K via ginsenoside F2, and inner glucosidic linkage at the C-3 site of compound Mc1 to produce ginsenoside compound Mc. Compound Mc is also slowly hydrolyzes alpha-(1->6)-arabinofuranosidic linkage at the C-20 site to produce compound K with reaction time prolongation. Finally, the pathways for formation of C-Mc and C-K from G-Rc by enzyme BG-1 are G-Rc -> C-Mc1 -> C-Mc and G-Rc -> G-Rd -> G-F2 -> C-K, respectively. Compound identification by TLC, HPLC, NMR spectroscopic, and UPLC/Q-TOF-MS analysis, detailed overview. No activity with 4-nitrophenyl -alpha-D-glucopyranoside and 4-nitrophenyl-alpha- and -beta-galactopyranoside Armillaria mellea KACC 50013 ?
-
 ?
3.2.1.193 additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Pleurotus ostreatus KACC 50356 ?
-
 ?
3.2.1.193 additional information the enzyme from hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Ganoderma applanatum KACC 53688 ?
-
 ?
3.2.1.193 additional information the enzyme hydrolyzes selectively alpha-(1->6)-arabinofuranosidic linkage at the C-20 position of ginsenoside Rc without attacking any other beta-glucosidic and arabinofuranosidic linkages Sanghuangporus baumii KACC 53719 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.193 ? x * 121500, SDS-PAGE Armillaria mellea

Synonyms

EC Number Synonyms Comment Organism
3.2.1.193 BG-1
-
 Armillaria mellea
3.2.1.193 ginsenoside Rc-hydrolyzing beta-glucosidase
-
 Pleurotus ostreatus
3.2.1.193 ginsenoside Rc-hydrolyzing beta-glucosidase
-
 Ganoderma lucidum
3.2.1.193 ginsenoside Rc-hydrolyzing beta-glucosidase
-
 Armillaria mellea
3.2.1.193 ginsenoside Rc-hydrolyzing beta-glucosidase
-
 Ganoderma applanatum
3.2.1.193 ginsenoside Rc-hydrolyzing beta-glucosidase
-
 Sanghuangporus baumii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.193 45 50 the optimum temperature for compound Mc and compound K formation are slightly different, 55-60°C for C-Mc and 45-50°C for compound K Armillaria mellea
3.2.1.193 55 60 the optimum temperature for compound Mc and compound K formation are slightly different, 55-60°C for C-Mc and 45-50°C for compound K Armillaria mellea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.193 4 4.5
-
 Armillaria mellea

General Information

EC Number General Information Comment Organism
3.2.1.193 evolution Armillaria mellea beta-glucosidase exists in three isomeric forms, BG-1, BG-2 and BG-3, that they exhibit different retention behaviors on DEAE cellulose column and hydrolytic activity toward 4-nitrophenyl beta-D-glucopyranoside. Moreover, when ginsenosides Rc, Rb1 andRd are used as substrates, isozyme BG-1 shows different hydrolytic activity from those of isozymes BG-2 and BG-3 Armillaria mellea