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Literature summary extracted from

  • Ueda, J.; Kurosawa, N.
    Characterization of an extracellular thermophilic chitinase from Paenibacillus thermoaerophilus strain TC22-2b isolated from compost (2015), World J. Microbiol. Biotechnol., 31, 135-143 .
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 1.4
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside at pH 7.0 and 60°C Paenibacillus thermoaerophilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular
-
 Paenibacillus thermoaerophilus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.14 colloidal chitin + H2O Paenibacillus thermoaerophilus
-
 N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
-
 ?
3.2.1.14 colloidal chitin + H2O Paenibacillus thermoaerophilus TC22-2b
-
 N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Paenibacillus thermoaerophilus
-
-
-
3.2.1.14 Paenibacillus thermoaerophilus TC22-2b
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 ammonium sulfate precipitation, colloidal chitin adsorption, and DEAE Sepharose column chromatography Paenibacillus thermoaerophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Paenibacillus thermoaerophilus 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Paenibacillus thermoaerophilus TC22-2b 4-nitrophenol + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Paenibacillus thermoaerophilus N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
-
 ?
3.2.1.14 colloidal chitin + H2O
-
 Paenibacillus thermoaerophilus TC22-2b N,N'-diacetyl-beta-D-chitobiose + N,N',N'''-triacetyl-beta-D-chitotriose + N,N',N'',N'''-tetracetyl-beta-D-chitotetraose
-
 ?
3.2.1.14 additional information the enzyme does not hydrolyze N,N'-diacetyl-beta-D-chitobiose Paenibacillus thermoaerophilus ?
-
 ?
3.2.1.14 additional information the enzyme does not hydrolyze N,N'-diacetyl-beta-D-chitobiose Paenibacillus thermoaerophilus TC22-2b ?
-
 ?
3.2.1.14 N,N',N'''-triacetyl-beta-D-chitotriose + H2O
-
 Paenibacillus thermoaerophilus N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 N,N',N'''-triacetyl-beta-D-chitotriose + H2O
-
 Paenibacillus thermoaerophilus TC22-2b N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O
-
 Paenibacillus thermoaerophilus N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-hexacetyl-beta-D-chitohexaose + H2O
-
 Paenibacillus thermoaerophilus TC22-2b N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 N,N',N'',N''',N'''',N'''''-pentacetyl-beta-D-chitopentaose + H2O
-
 Paenibacillus thermoaerophilus N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose
-
 ?
3.2.1.14 N,N',N'',N'''-tetracetyl-beta-D-chitotetraose + H2O
-
 Paenibacillus thermoaerophilus N,N'-diacetyl-beta-D-chitobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 48000, SDS-PAGE Paenibacillus thermoaerophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
-
 Paenibacillus thermoaerophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 50 70 more than 69% of the maximum activity is observed in the temperature range of 50-70°C. Enzyme activity increases from 30 to 60°C and then declined markedly at temperature of more than 70°C Paenibacillus thermoaerophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 30 80 the enzyme has no activity when incubated at 80°C for 2 h. However, it retains more than 68 % of its initial activity after incubation at 30, 40, and 50°C for 2 h. This enzyme also retains more than 47 % of its initial activity after incubation at 30, 40, and 50°C for 24 h Paenibacillus thermoaerophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 9.6
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside at pH 7.0 and 60°C Paenibacillus thermoaerophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 4
-
-
 Paenibacillus thermoaerophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 7 more than 50% of its maximum activity is observed in the pH range of 4.0-7.0 Paenibacillus thermoaerophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 3 10 the enzyme retains more than 80% activity after incubation at 30°C for 2 h in the pH range of 4.0-10.0. Residual activity is reduced at an acidic pH, in particular, approximately 60% of its activity is lost at pH 3.0 Paenibacillus thermoaerophilus

Expression

EC Number Organism Comment Expression
3.2.1.14 Paenibacillus thermoaerophilus adding D-glucose, lactose, D-mannose, or sucrose to culture medium decreases the amount of the enzyme down

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.14 6.8
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside at pH 7.0 and 60°C Paenibacillus thermoaerophilus