Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Megson, Z.A.; Koerdt, A.; Schuster, H.; Ludwig, R.; Janesch, B.; Frey, A.; Naylor, K.; Wilson, I.B.; Stafford, G.P.; Messner, P.; Schaeffer, C.
    Characterization of an alpha-L-fucosidase from the periodontal pathogen Tannerella forsythia (2015), Virulence, 6, 282-292 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 the Tffuc1 gene is cloned into pET22-b(C) vector and expressed in Escherichia coli as a C-terminally His6-tagged protein Tannerella forsythia
3.2.1.63 expressed in Escherichia coli BL21(DE3) cells Tannerella forsythia

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 alpha-L-fucose competitive Tannerella forsythia
3.2.1.51 deoxyfuconojirimycin competitive Tannerella forsythia
3.2.1.63 deoxyfuconojirimycin complete inhibition at 0.0001 mM Tannerella forsythia
3.2.1.63 L-fucose complete inhibition at 0.25 mM Tannerella forsythia
3.2.1.127 deoxyfuconojirimycin competitive Tannerella forsythia
3.2.1.127 fucose competitive Tannerella forsythia

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.67
-
 4-nitrophenyl alpha-L-fucopyranoside 22°C, pH 9.0 Tannerella forsythia
3.2.1.63 0.67
-
 4-nitrophenyl alpha-L-fucopyranoside at pH 9.0 and 22°C Tannerella forsythia
3.2.1.127 0.67
-
 4-nitrophenyl alpha-L-fucopyranoside 22°C, pH 9.0 Tannerella forsythia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.51 additional information all the detectable activity is found in the non-membrane associated fraction comprising both the cytoplasmic and periplasmic content Tannerella forsythia
-
-
3.2.1.63 membrane
-
 Tannerella forsythia 16020
-
3.2.1.127 additional information following knock-out of the Tffuc1 gene and analyzing biofilm formation and cell invasion/adhesion of the mutant in comparison to the wild-type, it is most likely that the enzyme does not act extracellularly Tannerella forsythia
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.127 51000
-
-
 Tannerella forsythia

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Tannerella forsythia G8UMQ6
-
-
3.2.1.51 Tannerella forsythia ATCC 43037 G8UMQ6
-
-
3.2.1.63 Tannerella forsythia
-
-
-
3.2.1.63 Tannerella forsythia ATCC 43037
-
-
-
3.2.1.127 Tannerella forsythia G8UMQ6
-
-
3.2.1.127 Tannerella forsythia ATCC 43037 G8UMQ6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Tannerella forsythia
3.2.1.63 Ni-NTA bead chromatography Tannerella forsythia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia 4-nitrophenol + alpha-L-fucopyranose
-
 ?
3.2.1.51 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia ATCC 43037 4-nitrophenol + alpha-L-fucopyranose
-
 ?
3.2.1.51 additional information TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ?
-
 ?
3.2.1.51 additional information TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ATCC 43037 ?
-
 ?
3.2.1.63 2'-fucosyllactose + H2O
-
 Tannerella forsythia L-fucose + lactose
-
 ?
3.2.1.63 2'-fucosyllactose + H2O
-
 Tannerella forsythia ATCC 43037 L-fucose + lactose
-
 ?
3.2.1.63 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia 4-nitrophenol + L-fucopyranose
-
 ?
3.2.1.63 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia ATCC 43037 4-nitrophenol + L-fucopyranose
-
 ?
3.2.1.63 alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O the enzyme also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ?
-
 ?
3.2.1.63 alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O the enzyme also possesses alpha(1,6) specificity on small unbranched substrates Tannerella forsythia ATCC 43037 ?
-
 ?
3.2.1.63 bovine submaxillary mucin + H2O
-
 Tannerella forsythia L-fucose + ?
-
 ?
3.2.1.63 bovine submaxillary mucin + H2O
-
 Tannerella forsythia ATCC 43037 L-fucose + ?
-
 ?
3.2.1.63 additional information the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal Tannerella forsythia ?
-
 ?
3.2.1.63 additional information the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal Tannerella forsythia ATCC 43037 ?
-
 ?
3.2.1.127 2'-fucosyl-N-acetyllactosamine + H2O
-
 Tannerella forsythia alpha-L-fucose + ?
-
 ?
3.2.1.127 2'-fucosyl-N-acetyllactosamine + H2O
-
 Tannerella forsythia ATCC 43037 alpha-L-fucose + ?
-
 ?
3.2.1.127 2-fucosyllactose + H2O
-
 Tannerella forsythia alpha-L-fucose + lactose
-
 ?
3.2.1.127 2-fucosyllactose + H2O
-
 Tannerella forsythia ATCC 43037 alpha-L-fucose + lactose
-
 ?
3.2.1.127 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Tannerella forsythia 4-nitrophenol + alpha-L-fucopyranose
-
 ?
3.2.1.127 alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
-
 Tannerella forsythia alpha-L-fucose + N-acetyl-D-glucosamine
-
 ?
3.2.1.127 additional information the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal Tannerella forsythia ?
-
 ?
3.2.1.127 additional information the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal Tannerella forsythia ATCC 43037 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.63 ? x * 51000, SDS-PAGE Tannerella forsythia

Synonyms

EC Number Synonyms Comment Organism
3.2.1.51 bfo_2737
-
 Tannerella forsythia
3.2.1.51 TfFuc1
-
 Tannerella forsythia
3.2.1.63 alpha(1,2) fucosidase
-
 Tannerella forsythia
3.2.1.63 bfo_2737
-
 Tannerella forsythia
3.2.1.63 Fuc1
-
 Tannerella forsythia
3.2.1.127 bfo_2737
-
 Tannerella forsythia
3.2.1.127 TfFuc1
-
 Tannerella forsythia

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 9
-
-
 Tannerella forsythia
3.2.1.63 9
-
-
 Tannerella forsythia
3.2.1.127 9
-
-
 Tannerella forsythia

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.127 8 10.2 pH 8.0: about 70% of maximal activity, pH 10.2: about 40% of maximal activity Tannerella forsythia

General Information

EC Number General Information Comment Organism
3.2.1.51 physiological function the biological role of the enzyme may well be in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism Tannerella forsythia
3.2.1.63 physiological function the enzyme contributes to the virulence of Tannerella forsythia Tannerella forsythia
3.2.1.127 physiological function the biological role of the enzyme in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism Tannerella forsythia