BRENDA - Enzyme Database

Characterization of an alpha-L-fucosidase from the periodontal pathogen Tannerella forsythia

Megson, Z.A.; Koerdt, A.; Schuster, H.; Ludwig, R.; Janesch, B.; Frey, A.; Naylor, K.; Wilson, I.B.; Stafford, G.P.; Messner, P.; Schaeffer, C.; Virulence 6, 282-292 (2015)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.2.1.51
the Tffuc1 gene is cloned into pET22-b(C) vector and expressed in Escherichia coli as a C-terminally His6-tagged protein
Tannerella forsythia
3.2.1.63
expressed in Escherichia coli BL21(DE3) cells
Tannerella forsythia
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive
Tannerella forsythia
3.2.1.51
deoxyfuconojirimycin
competitive
Tannerella forsythia
3.2.1.63
deoxyfuconojirimycin
complete inhibition at 0.0001 mM
Tannerella forsythia
3.2.1.63
L-fucose
complete inhibition at 0.25 mM
Tannerella forsythia
3.2.1.127
deoxyfuconojirimycin
competitive
Tannerella forsythia
3.2.1.127
fucose
competitive
Tannerella forsythia
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.67
-
4-nitrophenyl alpha-L-fucopyranoside
22°C, pH 9.0
Tannerella forsythia
3.2.1.63
0.67
-
4-nitrophenyl alpha-L-fucopyranoside
at pH 9.0 and 22°C
Tannerella forsythia
3.2.1.127
0.67
-
4-nitrophenyl alpha-L-fucopyranoside
22°C, pH 9.0
Tannerella forsythia
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
additional information
all the detectable activity is found in the non-membrane associated fraction comprising both the cytoplasmic and periplasmic content
Tannerella forsythia
-
-
3.2.1.63
membrane
-
Tannerella forsythia
16020
-
3.2.1.127
additional information
following knock-out of the Tffuc1 gene and analyzing biofilm formation and cell invasion/adhesion of the mutant in comparison to the wild-type, it is most likely that the enzyme does not act extracellularly
Tannerella forsythia
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.127
51000
-
-
Tannerella forsythia
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Tannerella forsythia
G8UMQ6
-
-
3.2.1.51
Tannerella forsythia ATCC 43037
G8UMQ6
-
-
3.2.1.63
Tannerella forsythia
-
-
-
3.2.1.63
Tannerella forsythia ATCC 43037
-
-
-
3.2.1.127
Tannerella forsythia
G8UMQ6
-
-
3.2.1.127
Tannerella forsythia ATCC 43037
G8UMQ6
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
-
Tannerella forsythia
3.2.1.63
Ni-NTA bead chromatography
Tannerella forsythia
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia ATCC 43037
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
additional information
TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia
?
-
-
-
-
3.2.1.51
additional information
TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia ATCC 43037
?
-
-
-
-
3.2.1.63
2'-fucosyllactose + H2O
-
752322
Tannerella forsythia
L-fucose + lactose
-
-
-
?
3.2.1.63
2'-fucosyllactose + H2O
-
752322
Tannerella forsythia ATCC 43037
L-fucose + lactose
-
-
-
?
3.2.1.63
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia
4-nitrophenol + L-fucopyranose
-
-
-
?
3.2.1.63
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia ATCC 43037
4-nitrophenol + L-fucopyranose
-
-
-
?
3.2.1.63
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
the enzyme also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia
?
-
-
-
?
3.2.1.63
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
the enzyme also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia ATCC 43037
?
-
-
-
?
3.2.1.63
bovine submaxillary mucin + H2O
-
752322
Tannerella forsythia
L-fucose + ?
-
-
-
?
3.2.1.63
bovine submaxillary mucin + H2O
-
752322
Tannerella forsythia ATCC 43037
L-fucose + ?
-
-
-
?
3.2.1.63
additional information
the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal
752322
Tannerella forsythia
?
-
-
-
-
3.2.1.63
additional information
the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal
752322
Tannerella forsythia ATCC 43037
?
-
-
-
-
3.2.1.127
2'-fucosyl-N-acetyllactosamine + H2O
-
752322
Tannerella forsythia
alpha-L-fucose + ?
-
-
-
?
3.2.1.127
2'-fucosyl-N-acetyllactosamine + H2O
-
752322
Tannerella forsythia ATCC 43037
alpha-L-fucose + ?
-
-
-
?
3.2.1.127
2-fucosyllactose + H2O
-
752322
Tannerella forsythia
alpha-L-fucose + lactose
-
-
-
?
3.2.1.127
2-fucosyllactose + H2O
-
752322
Tannerella forsythia ATCC 43037
alpha-L-fucose + lactose
-
-
-
?
3.2.1.127
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.127
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
-
752322
Tannerella forsythia
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
3.2.1.127
additional information
the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal
752322
Tannerella forsythia
?
-
-
-
-
3.2.1.127
additional information
the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal
752322
Tannerella forsythia ATCC 43037
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.63
?
x * 51000, SDS-PAGE
Tannerella forsythia
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
9
-
-
Tannerella forsythia
3.2.1.63
9
-
-
Tannerella forsythia
3.2.1.127
9
-
-
Tannerella forsythia
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.127
8
10.2
pH 8.0: about 70% of maximal activity, pH 10.2: about 40% of maximal activity
Tannerella forsythia
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
the Tffuc1 gene is cloned into pET22-b(C) vector and expressed in Escherichia coli as a C-terminally His6-tagged protein
Tannerella forsythia
3.2.1.63
expressed in Escherichia coli BL21(DE3) cells
Tannerella forsythia
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competitive
Tannerella forsythia
3.2.1.51
deoxyfuconojirimycin
competitive
Tannerella forsythia
3.2.1.63
deoxyfuconojirimycin
complete inhibition at 0.0001 mM
Tannerella forsythia
3.2.1.63
L-fucose
complete inhibition at 0.25 mM
Tannerella forsythia
3.2.1.127
deoxyfuconojirimycin
competitive
Tannerella forsythia
3.2.1.127
fucose
competitive
Tannerella forsythia
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.67
-
4-nitrophenyl alpha-L-fucopyranoside
22°C, pH 9.0
Tannerella forsythia
3.2.1.63
0.67
-
4-nitrophenyl alpha-L-fucopyranoside
at pH 9.0 and 22°C
Tannerella forsythia
3.2.1.127
0.67
-
4-nitrophenyl alpha-L-fucopyranoside
22°C, pH 9.0
Tannerella forsythia
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.2.1.51
additional information
all the detectable activity is found in the non-membrane associated fraction comprising both the cytoplasmic and periplasmic content
Tannerella forsythia
-
-
3.2.1.63
membrane
-
Tannerella forsythia
16020
-
3.2.1.127
additional information
following knock-out of the Tffuc1 gene and analyzing biofilm formation and cell invasion/adhesion of the mutant in comparison to the wild-type, it is most likely that the enzyme does not act extracellularly
Tannerella forsythia
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.127
51000
-
-
Tannerella forsythia
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
-
Tannerella forsythia
3.2.1.63
Ni-NTA bead chromatography
Tannerella forsythia
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia ATCC 43037
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.51
additional information
TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia
?
-
-
-
-
3.2.1.51
additional information
TfFuc1 is a unique alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia ATCC 43037
?
-
-
-
-
3.2.1.63
2'-fucosyllactose + H2O
-
752322
Tannerella forsythia
L-fucose + lactose
-
-
-
?
3.2.1.63
2'-fucosyllactose + H2O
-
752322
Tannerella forsythia ATCC 43037
L-fucose + lactose
-
-
-
?
3.2.1.63
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia
4-nitrophenol + L-fucopyranose
-
-
-
?
3.2.1.63
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia ATCC 43037
4-nitrophenol + L-fucopyranose
-
-
-
?
3.2.1.63
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
the enzyme also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia
?
-
-
-
?
3.2.1.63
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
the enzyme also possesses alpha(1,6) specificity on small unbranched substrates
752322
Tannerella forsythia ATCC 43037
?
-
-
-
?
3.2.1.63
bovine submaxillary mucin + H2O
-
752322
Tannerella forsythia
L-fucose + ?
-
-
-
?
3.2.1.63
bovine submaxillary mucin + H2O
-
752322
Tannerella forsythia ATCC 43037
L-fucose + ?
-
-
-
?
3.2.1.63
additional information
the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal
752322
Tannerella forsythia
?
-
-
-
-
3.2.1.63
additional information
the alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on A antigen tetraose and alpha-L-Fuc-(1->4)-beta-D-Gal
752322
Tannerella forsythia ATCC 43037
?
-
-
-
-
3.2.1.127
2'-fucosyl-N-acetyllactosamine + H2O
-
752322
Tannerella forsythia
alpha-L-fucose + ?
-
-
-
?
3.2.1.127
2'-fucosyl-N-acetyllactosamine + H2O
-
752322
Tannerella forsythia ATCC 43037
alpha-L-fucose + ?
-
-
-
?
3.2.1.127
2-fucosyllactose + H2O
-
752322
Tannerella forsythia
alpha-L-fucose + lactose
-
-
-
?
3.2.1.127
2-fucosyllactose + H2O
-
752322
Tannerella forsythia ATCC 43037
alpha-L-fucose + lactose
-
-
-
?
3.2.1.127
4-nitrophenyl alpha-L-fucopyranoside + H2O
-
752322
Tannerella forsythia
4-nitrophenol + alpha-L-fucopyranose
-
-
-
?
3.2.1.127
alpha-L-Fuc-(1->6)-beta-D-GlcNAc + H2O
-
752322
Tannerella forsythia
alpha-L-fucose + N-acetyl-D-glucosamine
-
-
-
?
3.2.1.127
additional information
the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal
752322
Tannerella forsythia
?
-
-
-
-
3.2.1.127
additional information
the enzyme is a alpha(1,2)-fucosidase that also possesses alpha(1,6) specificity on small unbranched substrates. It is active on mucin after sialidase-catalyzed removal of terminal sialic acid residues and also removes fucose from blood group H. The alpha(1,3) and alpha(1,4) linkages are not cleaved on 3-fucosyllactose and the Lewis A trisaccharide, respectively. The enzyme is also inactive on the substrate alpha-L-Fuc-(1,4)-beta-D-Gal
752322
Tannerella forsythia ATCC 43037
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.63
?
x * 51000, SDS-PAGE
Tannerella forsythia
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.2.1.51
9
-
-
Tannerella forsythia
3.2.1.63
9
-
-
Tannerella forsythia
3.2.1.127
9
-
-
Tannerella forsythia
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.2.1.127
8
10.2
pH 8.0: about 70% of maximal activity, pH 10.2: about 40% of maximal activity
Tannerella forsythia
General Information
EC Number
General Information
Commentary
Organism
3.2.1.51
physiological function
the biological role of the enzyme may well be in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism
Tannerella forsythia
3.2.1.63
physiological function
the enzyme contributes to the virulence of Tannerella forsythia
Tannerella forsythia
3.2.1.127
physiological function
the biological role of the enzyme in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism
Tannerella forsythia
General Information (protein specific)
EC Number
General Information
Commentary
Organism
3.2.1.51
physiological function
the biological role of the enzyme may well be in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism
Tannerella forsythia
3.2.1.63
physiological function
the enzyme contributes to the virulence of Tannerella forsythia
Tannerella forsythia
3.2.1.127
physiological function
the biological role of the enzyme in the metabolism of short oligosaccharides in the periplasm, thereby indirectly contributing to the virulence of this organism
Tannerella forsythia