EC Number | Application | Comment | Organism |
---|---|---|---|
3.1.1.74 | synthesis | expression of enzyme in Saccharomyces cerevisiae. Approximately 28% of the cutinase localizes to the cell walls and/or between cell wall and cell membrane. Protoplasts entrapped in a membrane capsule with a low-viscous liquid-core of 1.92 % w/v calcium-alginate in a static culture secrete measurable amounts of cutinase into the broth. The entrapped protoplasts are cultivated in a shake flask at low osmotic pressure without disruption. During 60 h of cultivation, the extracellular cutinase activity of the free protoplasts at 29.3 atm and protoplasts entrapped in the capsule at 17.2 atm are 0.13 and 0.39 U/mL, respectively | Aspergillus oryzae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.1.74 | expression in Saccharomyces cerevisiae | Aspergillus oryzae |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.1.74 | Aspergillus oryzae | P52956 | - |
- |
3.1.1.74 | Aspergillus oryzae ATCC 42149 | P52956 | - |
- |