Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Morita, J.; Kano, K.; Kato, K.; Takita, H.; Sakagami, H.; Yamamoto, Y.; Mihara, E.; Ueda, H.; Sato, T.; Tokuyama, H.; Arai, H.; Asou, H.; Takagi, J.; Ishitani, R.; Nishimasu, H.; Nureki, O.; Aoki, J.
    Structure and biological function of ENPP6, a choline-specific glycerophosphodiester-phosphodiesterase (2016), Sci. Rep., 6, 20995 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.2 expressed in HEK-293T cells Mus musculus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.4.2 C393A/C412S mutant apoenzyme in complex with sn-glycero-3-phosphocholine, vapor diffusion method, using 0.2 M NH4Cl, 0.1 M sodium acetate pH 5.0, 20% (w/v) PEG 6000 Mus musculus
3.1.4.38 structure of ENPP6 in complex with phosphocholine. The choline moiety of the phosphocholine is recognized by a choline-binding pocket formed by conserved aromatic and acidic residues Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.2 C154A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
3.1.4.2 C393A/C412S the mutations do not affect enzyme activity Mus musculus
3.1.4.2 E143A the mutant enzyme shows about 50% reduced activity compared to the wild type enzyme Mus musculus
3.1.4.2 E190A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
3.1.4.2 S71A the mutant enzyme is almost inactive Mus musculus
3.1.4.2 S71T inactive Mus musculus
3.1.4.2 Y188A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
3.1.4.2 Y72A the mutant enzyme is almost inactive Mus musculus
3.1.4.2 Y75A the mutant enzyme shows severely reduced activity compared to the wild type enzyme Mus musculus
3.1.4.2 Y75A/Y188A inactive Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.4.2 nucleus
-
 Mus musculus 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.2 Zn2+ the enzyme contains 2 Zn2+ ions Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.2 sn-glycero-3-phosphocholine + H2O Mus musculus specific substrate choline + sn-glycerol 3-phosphate
-
 ?
3.1.4.38 sn-glycero-3-phosphocholine + H2O Mus musculus the choline moiety of glycero-3-phosphocholine is incorporated into phosphocholine in an ENPP6-dependent manner both in vivo and in vitro glycerol + phosphocholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.2 Mus musculus Q8BGN3
-
-
3.1.4.38 Mus musculus Q8BGN3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.2
-
 Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.2 brain
-
 Mus musculus
-
3.1.4.2 heart
-
 Mus musculus
-
3.1.4.2 kidney
-
 Mus musculus
-
3.1.4.2 liver sinusoidal endothelial cell
-
 Mus musculus
-
3.1.4.2 lung
-
 Mus musculus
-
3.1.4.2 oligodendrocyte developing oligodendrocytes Mus musculus
-
3.1.4.2 spleen
-
 Mus musculus
-
3.1.4.38 liver high expression in liver sinusoidal endothelial cells Mus musculus
-
3.1.4.38 oligodendrocyte high expression Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.2 sn-glycero-3-phosphocholine + H2O
-
 Mus musculus choline + sn-glycerol 3-phosphate
-
 ?
3.1.4.2 sn-glycero-3-phosphocholine + H2O specific substrate Mus musculus choline + sn-glycerol 3-phosphate
-
 ?
3.1.4.38 sn-glycero-3-phosphocholine + H2O
-
 Mus musculus glycerol + phosphocholine
-
 ?
3.1.4.38 sn-glycero-3-phosphocholine + H2O the choline moiety of glycero-3-phosphocholine is incorporated into phosphocholine in an ENPP6-dependent manner both in vivo and in vitro Mus musculus glycerol + phosphocholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.2 ? x * 50000, SDS-PAGE Mus musculus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.2 choline-specific glycerophosphodiester-phosphodiesterase
-
 Mus musculus
3.1.4.2 ENPP6
-
 Mus musculus
3.1.4.38 ectonucleotide pyrophosphatase/phosphodiesterase family member 6
-
 Mus musculus
3.1.4.38 ENPP6
-
 Mus musculus

General Information

EC Number General Information Comment Organism
3.1.4.2 malfunction enzyme-deficient mice exhibit fatty liver and hypomyelination Mus musculus
3.1.4.2 metabolism the enzyme participates in choline metabolism Mus musculus
3.1.4.38 physiological function ENPP6-deficient mice exhibit fatty liver and hypomyelination Mus musculus