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Literature summary extracted from

  • Habash, M.; Chu, B.C.; Trevors, J.T.; Lee, H.
    Mutational study of the role of N-terminal amino acid residues in tetrachlorohydroquinone reductive dehalogenase from Sphingomonas sp. UG30 (2009), Res. Microbiol., 160, 553-559 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.21.4.5 expression in Escherichia coli Sphingobium sp. UG30

Protein Variants

EC Number Protein Variants Comment Organism
1.21.4.5 C14A the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 44°C for mutant enzyme S12A Sphingobium sp. UG30
1.21.4.5 S12A the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme, the mutant enzyme shows an increase in the molar ellipticity between 260 and 280 nm, relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 44°C for mutant enzyme S12A Sphingobium sp. UG30
1.21.4.5 S15A the mutant enzyme displays greater than 25fold decreased activity relative to the wild-type enzyme. The pH optimum decreases from 50°C for the wild-type enzyme to 40°C for mutant enzyme S12A Sphingobium sp. UG30
1.21.4.5 Y7F activity of the mutant enzyme is not significantly different from the wild-type enzyme Sphingobium sp. UG30
1.21.4.5 Y9F activity of the mutant enzyme is not significantly different from the wild-type enzyme Sphingobium sp. UG30

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.21.4.5 2,3,5,6-tetrachlorohydroquinone mutant enzymes S15A and S12A displayed substrate inhibition above 0.035 mM Sphingobium sp. UG30

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.21.4.5 11.4
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°, wild-type enzyme Sphingobium sp. UG30
1.21.4.5 16
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30
1.21.4.5 31.1
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
1.21.4.5 103
-
 glutathione pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
1.21.4.5 188
-
 glutathione pH 8.7, 37°C, wild-type enzyme Sphingobium sp. UG30
1.21.4.5 527
-
 glutathione pH 8.7, 37°C, mutant enzyme S15A Sphingobium sp. UG30
1.21.4.5 790
-
 glutathione pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.21.4.5 28000
-
 SDS-PAGE Sphingobium sp. UG30

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.21.4.5 2,3,5,6-tetrachlorohydroquinone + 2 glutathione Sphingobium sp. UG30
-
 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
1.21.4.5 2,3,6-trichlorohydroquinone + 2 glutathione Sphingobium sp. UG30
-
 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.21.4.5 Sphingobium sp. UG30 Q937X0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.21.4.5
-
 Sphingobium sp. UG30

Storage Stability

EC Number Storage Stability Organism
1.21.4.5 -20°C, enzyme activity is retained during storage under these conditions for at least 6 weeks Sphingobium sp. UG30

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.21.4.5 2,3,5,6-tetrachlorohydroquinone + 2 glutathione
-
 Sphingobium sp. UG30 2,3,6-trichlorohydroquinone + Cl- + glutathione disulfide
-
 ?
1.21.4.5 2,3,6-trichlorohydroquinone + 2 glutathione
-
 Sphingobium sp. UG30 2,6-dichlorohydroquinone + Cl- + glutathione disulfide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.21.4.5 ? x * 28000, SDS-PAGE Sphingobium sp. UG30

Synonyms

EC Number Synonyms Comment Organism
1.21.4.5 pcpC
-
 Sphingobium sp. UG30

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.21.4.5 40
-
 mutant enzyme S15A Sphingobium sp. UG30
1.21.4.5 44
-
 mutant enzyme S12A and mutant enzyme C14A Sphingobium sp. UG30
1.21.4.5 50
-
 wild-type enzyme Sphingobium sp. UG30

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.21.4.5 1.4
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30
1.21.4.5 6.3
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
1.21.4.5 9.6
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°, wild-type enzyme Sphingobium sp. UG30

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.21.4.5 0.087
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme C14A Sphingobium sp. UG30
1.21.4.5 0.204
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°C, mutant enzyme S12A Sphingobium sp. UG30
1.21.4.5 0.839
-
 2,3,5,6-tetrachlorohydroquinone pH 8.7, 37°, wild-type enzyme Sphingobium sp. UG30