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Literature summary extracted from
- Purification and characterization of alkaline phosphatase from water buffalo liver (Bubalus bubalis) (2016), Res. J. Pharm. Biol. Chem. Sci., 7, 1869-1880 .
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | 2-mercaptoethanol | the P2 isoenzyme is more sensitive to 2-mercaptoethanol than P1 | Bubalus bubalis |  |
| 3.1.3.1 | cysteine | - |
Bubalus bubalis | |
| 3.1.3.1 | dithiothreitol | - |
Bubalus bubalis | |
| 3.1.3.1 | EDTA | - |
Bubalus bubalis | |
| 3.1.3.1 | GSH | - |
Bubalus bubalis | |
| 3.1.3.1 | L-phenylalanine | the P2 isoenzyme is more sensitive to L-phenylalanine than P1 | Bubalus bubalis | |
| 3.1.3.1 | Zn2+ | potent inhibitor | Bubalus bubalis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.1 | Mn2+ | activates | Bubalus bubalis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 188000 | - |
isoenzyme P1, gel filtration | Bubalus bubalis |
| 3.1.3.1 | 194000 | - |
isoenzyme P2, gel filtration | Bubalus bubalis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | Bubalus bubalis | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.1 | DEAE-cellulose column chromatography and Sephacryl S-300 gel filtration | Bubalus bubalis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.3.1 | liver | - |
Bubalus bubalis | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.1 | 4-nitrophenyl phosphate + H2O | - |
Bubalus bubalis | 4-nitrophenol + phosphate | - |
? | |
| 3.1.3.1 | alpha-naphthyl phosphate + H2O | - |
Bubalus bubalis | alpha-naphthol + phosphate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.3.1 | homotrimer | - |
Bubalus bubalis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.1 | ALPase | - |
Bubalus bubalis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.1 | 10 | - |
- |
Bubalus bubalis |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.3.1 | Bubalus bubalis | isoelectric focusing | 5.8 | 5.6 |
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Release 2023.1 (January 2023)
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