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Literature summary extracted from

  • Schroeder, C.; Burkhardt, C.; Busch, P.; Schirrmacher, G.; Claren, J.; Antranikian, G.
    Characterization of a theme C glycoside hydrolase family 9 endo-beta-glucanase from a biogas reactor metagenome (2018), Protein J., 37, 454-460 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 gene bp_cel9A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 uncultured bacterium
3.2.1.73 gene bp_cel9A, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21 uncultured bacterium

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 EDTA 5 or 10 mM of EDTA reduce barley beta-glucan hydrolysis by 39% and 34%, respectively uncultured bacterium
3.2.1.4 EGTA 10 mM, 24% inhibition uncultured bacterium
3.2.1.4 Zn2+ 73% inhibition at 10 mM uncultured bacterium
3.2.1.73 EDTA 5 or 10 mM of EDTA reduce barley beta-glucan hydrolysis by 39% and 34%, respectively uncultured bacterium
3.2.1.73 EGTA 10 mM, 24% inhibition uncultured bacterium
3.2.1.73 Zn2+ 73% inhibition at 10 mM uncultured bacterium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ca2+ activates 40% at 5 mM and 31% at 10 mM uncultured bacterium
3.2.1.4 Mg2+ activates 31% at 5 mM uncultured bacterium
3.2.1.4 additional information the impact of metal ions Ca2+ and Mg2+ is more significant at higher temperatures uncultured bacterium
3.2.1.73 Ca2+ activates 40% at 5 mM and 31% at 10 mM uncultured bacterium
3.2.1.73 Mg2+ activates 31% at 5 mM uncultured bacterium
3.2.1.73 additional information the impact of metal ions Ca2+ and Mg2+ is more significant at higher temperatures uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 uncultured bacterium A0A346PYQ5
-
-
3.2.1.73 uncultured bacterium A0A346PYQ5
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.4 24
-
 purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.0, 30°C uncultured bacterium
3.2.1.73 24
-
 purified recombinant His-tagged enzyme, substrate barley beta-glucan, pH 6.0, 30°C uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 additional information the enzyme is active with beta-D-glucans containing (1->3)- and (1->4)-bonds (EC 3.2.1.73, licheninase) and with beta-D-glucans containing only (1->4)-bonds (EC 3.2.1.4, cellulase). It shows an exclusive endoacting mechanism. No activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-cellobioside, avicel, curdlan, laminarin, cellulose, carboxymethyl cellulose, and xylan uncultured bacterium ?
-
 ?
3.2.1.4 xyloglucan + H2O a beta-1,4-1,6-glucan, 40% activity compared to barley beta-glucan uncultured bacterium ?
-
 ?
3.2.1.73 barley beta-glucan + H2O endo-beta-glucanase BP_Cel9A hydrolyzes beta-1,3-1,4-linked barley beta-glucan, best substrate uncultured bacterium ?
-
 ?
3.2.1.73 lichenan + H2O 67% activity compared to barley beta-glucan uncultured bacterium ?
-
 ?
3.2.1.73 additional information the enzyme is active with beta-D-glucans containing (1->3)- and (1->4)-bonds (EC 3.2.1.73, licheninase) and with beta-D-glucans containing only (1->4)-bonds (EC 3.2.1.4, cellulase). It shows an exclusive endoacting mechanism. No activity with 4-nitrophenyl beta-D-glucopyranoside, 4-nitrophenyl beta-D-cellobioside, avicel, curdlan, laminarin, cellulose, carboxymethyl cellulose, and xylan uncultured bacterium ?
-
 ?
3.2.1.73 xyloglucan + H2O 40% activity compared to barley beta-glucan uncultured bacterium ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 62600, about, recombinant enzyme, sequence calculation uncultured bacterium
3.2.1.4 More domain organization, lack of a carbohydrate binding module uncultured bacterium
3.2.1.73 ? x * 62600, about, recombinant enzyme, sequence calculation uncultured bacterium
3.2.1.73 More domain organization, lack of a carbohydrate binding module uncultured bacterium

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 BP_Cel9A
-
 uncultured bacterium
3.2.1.4 endo-beta-glucanase
-
 uncultured bacterium
3.2.1.4 glycoside hydrolase family 9 endoglucanase
-
 uncultured bacterium
3.2.1.4 More cf. EC 3.2.1.73 uncultured bacterium
3.2.1.4 theme C glycoside hydrolase family 9 endo-beta-glucanase
-
 uncultured bacterium
3.2.1.73 BP_Cel9A
-
 uncultured bacterium
3.2.1.73 endo-beta-glucanase
-
 uncultured bacterium
3.2.1.73 glycoside hydrolase family 9 endoglucanase
-
 uncultured bacterium
3.2.1.73 More cf. EC 3.2.1.4 uncultured bacterium
3.2.1.73 theme C glycoside hydrolase family 9 endo-beta-glucanase
-
 uncultured bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 30
-
 assay at uncultured bacterium
3.2.1.73 30
-
 assay at uncultured bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 5 40 the enzyme shows 88% and 77% of maximal activity at 20°C and 40°C, and 63% and 38% of maximal activity at 10°C and at 5°C uncultured bacterium
3.2.1.73 5 40 the enzyme shows 88% and 77% of maximal activity at 20°C and 40°C, and 63% and 38% of maximal activity at 10°C and at 5°C uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6
-
 assay at uncultured bacterium
3.2.1.73 6
-
 assay at uncultured bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 6 8 maximal activity at pH 6.0, 95% activity at pH 7.0, and 66% activity at pH 8.0 uncultured bacterium
3.2.1.73 6 8 maximal activity at pH 6.0, 95% activity at pH 7.0, and 66% activity at pH 8.0 uncultured bacterium

General Information

EC Number General Information Comment Organism
3.2.1.4 evolution the enzyme belongs to the theme C glycoside hydrolase family 9, GH9. Theme C glucanases exhibit an N-terminal Ig domain uncultured bacterium
3.2.1.4 additional information the putative catalytic residues are Asp147, Asp150 and Glu517 uncultured bacterium
3.2.1.73 evolution the enzyme belongs to the theme C glycoside hydrolase family 9, GH9. Theme C glucanases exhibit an N-terminal Ig domain uncultured bacterium
3.2.1.73 additional information the putative catalytic residues are Asp147, Asp150 and Glu517 uncultured bacterium