Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Wahab, A.F.F.A.; Abdul Karim, N.A.; Ling, J.G.; Hasan, N.S.; Yong, H.Y.; Bharudin, I.; Kamaruddin, S.; Abu Bakar, F.D.; Murad, A.M.A.
    Functional characterisation of cellobiohydrolase I (Cbh1) from Trichoderma virens UKM1 expressed in Aspergillus niger (2019), Protein Expr. Purif., 154, 52-61 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.91 Tween 80 110.12% activity at 1% (v/v) Trichoderma virens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91 expressed in Aspergillus niger strain PY11 Trichoderma virens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.91 cellobiose competitive inhibitor Trichoderma virens
3.2.1.91 Co2+ 75.74% residual activity at 5 mM Trichoderma virens
3.2.1.91 Cu2+ 67.43% residual activity at 5 mM Trichoderma virens
3.2.1.91 D-glucose non-competitive inhibitor Trichoderma virens
3.2.1.91 EDTA 98.9% residual activity at 5 mM Trichoderma virens
3.2.1.91 K+ 90.66% residual activity at 5 mM Trichoderma virens
3.2.1.91 Mg2+ 68.12% residual activity at 5 mM Trichoderma virens
3.2.1.91 Na+ 96.61% residual activity at 5 mM Trichoderma virens
3.2.1.91 Ni2+ 70.55% residual activity at 5 mM Trichoderma virens
3.2.1.91 SDS 25.31% residual activity at 1% (w/v) Trichoderma virens
3.2.1.91 Zn2+ 91.5% residual activity at 5 mM Trichoderma virens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 1.88
-
 4-nitrophenyl beta-D-cellobioside at pH 4.0 and 60°C Trichoderma virens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.91 Ca2+ 107.14% activity at 5 mM Trichoderma virens
3.2.1.91 Mn2+ 113.88% activity at 5 mM Trichoderma virens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Trichoderma virens
-
-
-
3.2.1.91 Trichoderma virens UKM1
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.91 glycoprotein the enzyme has two potential N-glycosylation sites at Asn-283 and Asn-397 Trichoderma virens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 ammonium sulfate precipitation and anion exchange column chromatography Trichoderma virens

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.91 0.51
-
 crude enzyme, at pH 4.0 and 60°C Trichoderma virens
3.2.1.91 4.195
-
 after 8.22fold purification, at pH 4.0 and 60°C Trichoderma virens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Trichoderma virens 4-methylumbelliferone + cellobiose
-
 ?
3.2.1.91 4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Trichoderma virens UKM1 4-methylumbelliferone + cellobiose
-
 ?
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma virens 4-nitrophenol + cellobiose
-
 ?
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma virens UKM1 4-nitrophenol + cellobiose
-
 ?
3.2.1.91 4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Trichoderma virens 4-nitrophenol + beta-D-lactopyranose
-
 ?
3.2.1.91 4-nitrophenyl beta-D-lactopyranoside + H2O
-
 Trichoderma virens UKM1 4-nitrophenol + beta-D-lactopyranose
-
 ?
3.2.1.91 avicel + H2O
-
 Trichoderma virens ?
-
 ?
3.2.1.91 avicel + H2O
-
 Trichoderma virens UKM1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.91 ? x * 75000, recombinant enzyme, SDS-PAGE Trichoderma virens
3.2.1.91 ? x * 53280, calculated from amino acid sequence Trichoderma virens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 CBH1
-
 Trichoderma virens
3.2.1.91 cellobiohydrolase I
-
 Trichoderma virens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 60
-
-
 Trichoderma virens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.91 30 60 the enzyme is stable for 1 h up to 60°C Trichoderma virens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 4
-
-
 Trichoderma virens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.91 3 6 the enzyme is stable for 1 h at pH 3.0 to 6.0 in acetate buffer Trichoderma virens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.91 8.65
-
 cellobiose at pH 4.0 and 60°C Trichoderma virens
3.2.1.91 24.61
-
 D-glucose at pH 4.0 and 60°C Trichoderma virens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.91 56800
-
 4-nitrophenyl beta-D-cellobioside at pH 4.0 and 60°C Trichoderma virens