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Literature summary extracted from

  • Elena, C.; Cerminati, S.; Ravasi, P.; Rasia, R.; Peiru, S.; Menzella, H.; Castelli, M.
    B. cereus phospholipase C engineering for efficient degumming of vegetable oil (2017), Process Biochem., 54, 67-72 .
No PubMed abstract available

Application

EC Number Application Comment Organism
3.1.4.3 food industry the enzyme is used in vegetable oil refining by enzymatic phospholipid removal (degumming ) Bacillus cereus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.3 expressed in Pichia pastoris strain GS115H Bacillus cereus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.3 F66W the mutant enzyme can remove up to 90% of phosphatidylethanolamine while retaining its efficiency at hydrolyzing phosphatidylcholine Bacillus cereus
3.1.4.3 F66Y the mutant enzyme can remove up to 90% of phosphatidylethanolamine while retaining its efficiency at hydrolyzing phosphatidylcholine Bacillus cereus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 14.8
-
 4-nitrophenylphosphorylcholine mutant enzyme F66W, at pH 7.0 and 50°C Bacillus cereus
3.1.4.3 17.4
-
 4-nitrophenylphosphorylcholine mutant enzyme F66Y, at pH 7.0 and 50°C Bacillus cereus
3.1.4.3 39.5
-
 4-nitrophenylphosphorylcholine wild type enzyme, at pH 7.0 and 50°C Bacillus cereus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.3 Zn2+ contains 3 zinc ions Bacillus cereus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.3 phosphatidylcholine + H2O Bacillus cereus
-
 1,2-diacyl-sn-glycerol + phosphorylcholine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Bacillus cereus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 1,2-dihexanoyl-sn-glycero-3-phosphoethanolamine + H2O less efficient substrate Bacillus cereus 1,2-dihexanoyl-sn-glycerol + phosphoethanolamine
-
 ?
3.1.4.3 4-nitrophenylphosphorylcholine + H2O
-
 Bacillus cereus 4-nitrophenol + phosphorylcholine
-
 ?
3.1.4.3 phosphatidylcholine + H2O
-
 Bacillus cereus 1,2-diacyl-sn-glycerol + phosphorylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 monomer 1 * 28500, SDS-PAGE Bacillus cereus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.3 PLC
-
 Bacillus cereus
3.1.4.3 type C phospholipase
-
 Bacillus cereus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.4.3 3.9
-
 4-nitrophenylphosphorylcholine wild type enzyme, at pH 7.0 and 50°C Bacillus cereus
3.1.4.3 4.8
-
 4-nitrophenylphosphorylcholine mutant enzyme F66Y, at pH 7.0 and 50°C Bacillus cereus
3.1.4.3 5.4
-
 4-nitrophenylphosphorylcholine mutant enzyme F66W, at pH 7.0 and 50°C Bacillus cereus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1.4.3 98
-
 4-nitrophenylphosphorylcholine wild type enzyme, at pH 7.0 and 50°C Bacillus cereus
3.1.4.3 276
-
 4-nitrophenylphosphorylcholine mutant enzyme F66Y, at pH 7.0 and 50°C Bacillus cereus
3.1.4.3 365
-
 4-nitrophenylphosphorylcholine mutant enzyme F66W, at pH 7.0 and 50°C Bacillus cereus