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Literature summary extracted from

  • Weeks, A.M.; Wang, N.; Pelton, J.G.; Chang, M.C.Y.
    Entropy drives selective fluorine recognition in the fluoroacetyl-CoA thioesterase from Streptomyces cattleya (2018), Proc. Natl. Acad. Sci. USA, 115, E2193-E2201 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.1.2.29 F36A the mutation results in a change in KM by affecting substrate binding Streptomyces cattleya

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.2.29 acetyl-aza(dethia)-CoA
-
 Streptomyces cattleya
3.1.2.29 acetyl-carba(dethia)-CoA
-
 Streptomyces cattleya
3.1.2.29 acetyl-oxa(dethia)-CoA
-
 Streptomyces cattleya
3.1.2.29 fluoroacetyl-aza(dethia)-CoA
-
 Streptomyces cattleya
3.1.2.29 fluoroacetyl-carba(dethia)-CoA
-
 Streptomyces cattleya
3.1.2.29 fluoroacetyl-oxa(dethia)-CoA
-
 Streptomyces cattleya

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.2.29 fluoroacetyl-CoA + H2O Streptomyces cattleya
-
 fluoroacetate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.2.29 Streptomyces cattleya Q1EMV2
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.2.29 acetyl-CoA + H2O
-
 Streptomyces cattleya acetate + CoA
-
 ?
3.1.2.29 fluoroacetyl-CoA + H2O
-
 Streptomyces cattleya fluoroacetate + CoA
-
 ?
3.1.2.29 fluoroacetyl-CoA + H2O the enzyme is strongly selective for fluoroacetyl-CoA compared with acetyl-CoA Streptomyces cattleya fluoroacetate + CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.2.29 FlK
-
 Streptomyces cattleya
3.1.2.29 fluoroacetyl CoA thioesterase
-
 Streptomyces cattleya

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.2.29 0.13
-
 fluoroacetyl-oxa(dethia)-CoA wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 0.26
-
 fluoroacetyl-oxa(dethia)-CoA mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 0.3
-
 acetyl-oxa(dethia)-CoA mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 0.32
-
 fluoroacetyl-oxa(dethia)-CoA wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 0.33
-
 fluoroacetyl-oxa(dethia)-CoA mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 0.46
-
 fluoroacetyl-carba(dethia)-CoA wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 0.51
-
 acetyl-oxa(dethia)-CoA wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 0.9
-
 fluoroacetyl-carba(dethia)-CoA wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 1.2
-
 fluoroacetyl-aza(dethia)-CoA wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 1.5
-
 acetyl-oxa(dethia)-CoA mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 2.1
-
 fluoroacetyl-carba(dethia)-CoA mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 2.5
-
 acetyl-oxa(dethia)-CoA wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 2.6
-
 acetyl-carba(dethia)-CoA wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 2.8
-
 acetyl-aza(dethia)-CoA mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 3.6
-
 acetyl-carba(dethia)-CoA mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 4
-
 fluoroacetyl-aza(dethia)-CoA mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 4.3
-
 fluoroacetyl-aza(dethia)-CoA wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 4.5
-
 fluoroacetyl-carba(dethia)-CoA mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 4.7
-
 acetyl-aza(dethia)-CoA mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 7.5
-
 fluoroacetyl-aza(dethia)-CoA mutant enzyme F36A, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 8.4
-
 acetyl-aza(dethia)-CoA wild type enzyme, with acetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 11
-
 acetyl-carba(dethia)-CoA wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 33
-
 acetyl-aza(dethia)-CoA wild type enzyme, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya
3.1.2.29 51
-
 acetyl-carba(dethia)-CoA mutant enzyme F36A, with fluoroacetyl-CoA as substrate, at pH 7.6, temperature not specified in the publication Streptomyces cattleya