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Literature summary extracted from

  • Imada, K.; Minamino, T.; Uchida, Y.; Kinoshita, M.; Namba, K.
    Insight into the flagella type III export revealed by the complex structure of the type III ATPase and its regulator (2016), Proc. Natl. Acad. Sci. USA, 113, 3633-3638 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.4.2.8 expressed in Escherichia coli BL21(DE3) cells Salmonella enterica

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
7.4.2.8 FliHC2-FliI complex, sitting drop vapor diffusion method, using 0.1 M HEPES-NaOH (pH 7.2), 5% (w/v) PEG-400, and 0.1 M magnesium acetate Salmonella enterica

Protein Variants

EC Number Protein Variants Comment Organism
7.4.2.8 E16A the mutation slightly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
7.4.2.8 F15A the mutation slightly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
7.4.2.8 L12A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
7.4.2.8 R26A/R27A/R33A the mutations significantly reduce the binding affinity of the ATPase FliI for FliH Salmonella enterica
7.4.2.8 R4A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
7.4.2.8 R4A/R7A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
7.4.2.8 R7A the mutation slightly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica
7.4.2.8 W8A the mutation significantly reduces the binding affinity of the ATPase FliI for FliH Salmonella enterica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.4.2.8 cytoplasm
-
 Salmonella enterica 5737
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.4.2.8 ATP + H2O + cellular protein[side 1] Salmonella enterica
-
 ADP + phosphate + cellular protein[side 2]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.4.2.8 Salmonella enterica
-
 serovar typhimurium
-

Purification (Commentary)

EC Number Purification (Comment) Organism
7.4.2.8 Ni-NTA column chromatography Salmonella enterica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.4.2.8 ATP + H2O + cellular protein[side 1]
-
 Salmonella enterica ADP + phosphate + cellular protein[side 2]
-
 ?

Subunits

EC Number Subunits Comment Organism
7.4.2.8 homohexamer
-
 Salmonella enterica

Synonyms

EC Number Synonyms Comment Organism
7.4.2.8 FliI Walker-type ATPase component of the FliI6FliJ ATPase complex Salmonella enterica
7.4.2.8 FliI6FliJ complex FliI and FliJ form the FliI6FliJ ATPase complex of the bacterial flagellar export apparatus Salmonella enterica