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Literature summary extracted from

  • Arabaci, N.; Arikan, B.
    Isolation and characterization of a cold-active, alkaline, detergent stable alpha-amylase from a novel bacterium Bacillus subtilis N8 (2018), Prep. Biochem. Biotechnol., 48, 419-426 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.1 additional information poor effect of 2-mercaptoethanol Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ba2+
-
 Bacillus subtilis
3.2.1.1 Ca2+
-
 Bacillus subtilis
3.2.1.1 EDTA
-
 Bacillus subtilis
3.2.1.1 H2O2
-
 Bacillus subtilis
3.2.1.1 Mn2+
-
 Bacillus subtilis
3.2.1.1 Na+
-
 Bacillus subtilis
3.2.1.1 SDS
-
 Bacillus subtilis
3.2.1.1 Triton X-100
-
 Bacillus subtilis
3.2.1.1 Urea
-
 Bacillus subtilis
3.2.1.1 Zn2+
-
 Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 NaCl activates Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 205000
-
 native PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.1 starch + H2O Bacillus subtilis
-
 D-glucose + maltose + maltotriose
-
 ?
3.2.1.1 starch + H2O Bacillus subtilis N8
-
 D-glucose + maltose + maltotriose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Bacillus subtilis
-
 isolated from soil samples
-
3.2.1.1 Bacillus subtilis N8
-
 isolated from soil samples
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 additional information amylase synthesis in strain N8 optimally occurs at 15°C and pH 10.0 on agar plates containing starch. Optimization of growth conditions, overview Bacillus subtilis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 starch + H2O
-
 Bacillus subtilis D-glucose + maltose + maltotriose
-
 ?
3.2.1.1 starch + H2O
-
 Bacillus subtilis N8 D-glucose + maltose + maltotriose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 205000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 cold-active alpha-amylase
-
 Bacillus subtilis
3.2.1.1 endo-1,4-alpha-D-glucanohydrolase
-
 Bacillus subtilis
3.2.1.1 N8 alpha-amylase
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 25
-
-
 Bacillus subtilis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 10 40 activity range Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 25
-
 56% remaining activity after 24 h at pH 8.0, 50% after 24 h at pH 11.0-12.0 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 8
-
-
 Bacillus subtilis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 8
-
 56% remaining activity after 24 h at 25°C Bacillus subtilis
3.2.1.1 11 12 50% remaining activity after 24 h at 25°C Bacillus subtilis