EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.4 | biofuel production | the enzyme can be useful for production of bioethanol and biofuel | Fervidobacterium nodosum |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.4 | recombinant expression of FnCel5A in Escherichia coli strain BL21, method evaluation and optimization for overproduction and up-scaling to be used in production of bioethanol and biofuel | Fervidobacterium nodosum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | Fervidobacterium nodosum | D4PEB3 | - |
- |
3.2.1.4 | Fervidobacterium nodosum ATCC 35602 | D4PEB3 | - |
- |
3.2.1.4 | Fervidobacterium nodosum DSM 5306 | D4PEB3 | - |
- |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | additional information | - |
recombinant crude enzyme, 5.772 U/ml | Fervidobacterium nodosum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.4 | barley beta-glucan + H2O | high activity | Fervidobacterium nodosum | D-glucose + ? | - |
? | |
3.2.1.4 | barley beta-glucan + H2O | high activity | Fervidobacterium nodosum ATCC 35602 | D-glucose + ? | - |
? | |
3.2.1.4 | barley beta-glucan + H2O | high activity | Fervidobacterium nodosum DSM 5306 | D-glucose + ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | high activity | Fervidobacterium nodosum | D-glucose + ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | high activity | Fervidobacterium nodosum ATCC 35602 | D-glucose + ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | high activity | Fervidobacterium nodosum DSM 5306 | D-glucose + ? | - |
? | |
3.2.1.4 | cellulose + H2O | enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose | Fervidobacterium nodosum | D-glucose + ? | - |
? | |
3.2.1.4 | cellulose + H2O | enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose | Fervidobacterium nodosum ATCC 35602 | D-glucose + ? | - |
? | |
3.2.1.4 | cellulose + H2O | enzyme FnCel5A catalyzes the hydrolysis of cellulose to glucose | Fervidobacterium nodosum DSM 5306 | D-glucose + ? | - |
? | |
3.2.1.4 | additional information | the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan | Fervidobacterium nodosum | ? | - |
? | |
3.2.1.4 | additional information | the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan | Fervidobacterium nodosum ATCC 35602 | ? | - |
? | |
3.2.1.4 | additional information | the enzyme shows high affinity for carboxymethyl cellulose, but is also active with other substrates like beta-1,4-linked polysaccharides, including xyloglucan, glucomannan, beta-glucan, lichenin, and galactomannan | Fervidobacterium nodosum DSM 5306 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.4 | endoglucanase | - |
Fervidobacterium nodosum |
3.2.1.4 | FnCel5A | - |
Fervidobacterium nodosum |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 80 | - |
- |
Fervidobacterium nodosum |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 60 | - |
purified recombinant enzyme, half-life is 48 h | Fervidobacterium nodosum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.4 | 5.5 | - |
- |
Fervidobacterium nodosum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.2.1.4 | evolution | the enzyme belongs to the glycosyl hydrolase family 5, GH5 | Fervidobacterium nodosum |