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Literature summary extracted from

  • Park, S.; Yoo, W.; Lee, C.; Jeong, C.; Shin, S.; Kim, H.; Park, H.; Kim, K.; Kim, T.; Lee, J.
    Crystal structure and functional characterization of a cold-active acetyl xylan esterase (PbAcE) from psychrophilic soil microbe Paenibacillus sp. (2018), PLoS ONE, 13, e0206260 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.1.72 AcE forms a hexamer with a central substrate binding tunnel, and the intersubunit interactions are relatively weak. AcE also has a shorter loop and different residue composition in the beta4-alpha3 and beta5-alpha4 regions near the substrate binding site Paenibacillus sp. R4

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.72 234000
-
 analytical ultracentrifugation Paenibacillus sp. R4

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.72 Paenibacillus sp. R4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.72 4-nitrophenyl acetate + H2O
-
 Paenibacillus sp. R4 4-nitrophenol + acetate
-
 ?
3.1.1.72 7-amino cephalosporanic acid + H2O
-
 Paenibacillus sp. R4 ? + acetate
-
 ?
3.1.1.72 alpha-terpinyl acetate + H2O
-
 Paenibacillus sp. R4 alpha-terpineol + acetate
-
 ?
3.1.1.72 cefotaxime + H2O
-
 Paenibacillus sp. R4 ? + acetate
-
 ?
3.1.1.72 D-glucose penta-acetate + H2O
-
 Paenibacillus sp. R4 ? + acetate
-
 ?
3.1.1.72 linalyl acetate + H2O
-
 Paenibacillus sp. R4 linalool + acetate
-
 ?
3.1.1.72 additional information no substrates: cellulose acetate, N-acetyl glycosamine. The enzyme shows activity towards glyceryl tributanoate and glyceryl trioleate, olive oil and fish oil Paenibacillus sp. R4 ?
-
 ?
3.1.1.72 tertiary-butyl acetate + H2O
-
 Paenibacillus sp. R4 tert-butanol + acetate
-
 ?
3.1.1.72 xylan + acetate
-
 Paenibacillus sp. R4 xylan acetate
-
 ?
3.1.1.72 xylan acetate + H2O
-
 Paenibacillus sp. R4 xylan + acetate
-
 r

Subunits

EC Number Subunits Comment Organism
3.1.1.72 hexamer 6 * 36000, SDS-PAGE Paenibacillus sp. R4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.72 4
-
-
 Paenibacillus sp. R4

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.72 15
-
 about 92% of the activity at 4°C Paenibacillus sp. R4
3.1.1.72 25
-
 about 88% of the activity at 4°C Paenibacillus sp. R4