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Literature summary extracted from

  • Lezyk, M.; Jers, C.; Kjaerulff, L.; Gotfredsen, C.H.; Mikkelsen, M.D.; Mikkelsen, J.D.
    Novel alpha-L-fucosidases from a soil metagenome for production of fucosylated human milk oligosaccharides (2016), PLoS ONE, 11, e0147438 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.51 food industry the enzyme catalyse the transglycosylation reaction leading to production of fucosylated human milk oligosaccharides uncultured bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.51 alpha-L-fucosidase-encoding gene is identified by functional screening of a soil-derived metagenome library and expressed in Escherichia coli as recombinant 6xHis-tagged protein uncultured bacterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.071
-
 4-nitrophenyl alpha-L-fucopyranoside 30°C, pH 7.0 uncultured bacterium
3.2.1.51 0.11
-
 4-nitrophenyl alpha-L-fucopyranoside 30°C, pH 7.0 uncultured bacterium
3.2.1.51 0.13
-
 4-nitrophenyl alpha-L-fucopyranoside 30°C, pH 7.0 uncultured bacterium
3.2.1.51 0.14
-
 4-nitrophenyl alpha-L-fucopyranoside 30°C, pH 7.0 uncultured bacterium
3.2.1.51 0.28
-
 4-nitrophenyl alpha-L-fucopyranoside 30°C, pH 6.0 uncultured bacterium
3.2.1.51 0.31
-
 4-nitrophenyl alpha-L-fucopyranoside 30°C, pH 6.0 uncultured bacterium
3.2.1.51 0.4
-
 4-nitrophenyl alpha-L-fucopyranoside 30°C, pH 9.0 uncultured bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 uncultured bacterium A0A068B1E8 soil-derived metagenome
-
3.2.1.51 uncultured bacterium A0A068B2Y3 mfuc5; soil-derived metagenome
-
3.2.1.51 uncultured bacterium A0A068B3H5 soil-derived metagenome
-
3.2.1.51 uncultured bacterium A0A068B4L8 soil-derived metagenome
-
3.2.1.51 uncultured bacterium A0A068B4M2 soil-derived metagenome
-
3.2.1.51 uncultured bacterium A0A068B780 soil-derived metagenome
-
3.2.1.51 uncultured bacterium A0A068B783 soil-derived metagenome
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 uncultured bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 4-nitrophenyl alpha-L-fucopyranoside + H2O
-
 uncultured bacterium 4-nitrophenol + alpha-L-fucopyranose
-
 ?
3.2.1.51 additional information Mfuc5 is capable of both releasing fucose from citrus xyloglucan and and can also use it as a fucosyl-donor for synthesis of fucosyllactose uncultured bacterium ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.51 mfuc1
-
 uncultured bacterium
3.2.1.51 mfuc2
-
 uncultured bacterium
3.2.1.51 mfuc3
-
 uncultured bacterium
3.2.1.51 mfuc4
-
 uncultured bacterium
3.2.1.51 mfuc5
-
 uncultured bacterium
3.2.1.51 mfuc6
-
 uncultured bacterium
3.2.1.51 mfuc7
-
 uncultured bacterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 30
-
 half-life: 98 min uncultured bacterium
3.2.1.51 30
-
 half-life: above 2 h uncultured bacterium
3.2.1.51 40
-
 half-life: 28 min uncultured bacterium
3.2.1.51 40
-
 half-life: 56 min uncultured bacterium
3.2.1.51 40
-
 half-life: above 2 h uncultured bacterium
3.2.1.51 50
-
 half-life: 9 min uncultured bacterium
3.2.1.51 50
-
 half-life: 8 min uncultured bacterium
3.2.1.51 50
-
 half-life: 14 min uncultured bacterium
3.2.1.51 50
-
 half-life: 67 min uncultured bacterium
3.2.1.51 50
-
 half-life: above 2 h uncultured bacterium
3.2.1.51 50
-
 half-life: less than 2 min uncultured bacterium
3.2.1.51 60
-
 half-life: 4 min uncultured bacterium
3.2.1.51 60
-
 half-life: less than 2 min uncultured bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 6
-
-
 uncultured bacterium
3.2.1.51 7
-
-
 uncultured bacterium
3.2.1.51 9
-
-
 uncultured bacterium