Literature summary extracted from

Feng, Y.; Kitaoku, Y.; Tanaka, J.; Taira, T.; Ohnuma, T.; Aachmann, F.L.; Fukamizo, T.
Mode of action and specificity of a chitinase from unicellular microalgae, Euglena gracilis (2018), Plant Mol. Biol., 97, 553-564 .

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.14	catalytic domain Cat2 is expressed in Escherichia coli Rosetta-gami (DE3) cells	Euglena gracilis

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.14	Euglena gracilis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.14	HiTrap Q column chromatography and Sephacryl S-100 gel filtration	Euglena gracilis

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.14	beta-D-N,N',N'',N''',N'''',N'''''-hexacetylchitohexaose + H2O	-	Euglena gracilis	beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose	-	?
3.2.1.14	beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O	-	Euglena gracilis	beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose	-	?
3.2.1.14	beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O	-	Euglena gracilis	beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + N-acetyl-beta-D-glucosamine	-	?
3.2.1.14	chitosan + H2O	-	Euglena gracilis	?	-	?
3.2.1.14	additional information	the enzyme acts toward chitin polysaccharide chain through a cooperative action of the two endo-splitting catalytic domains, recognizing two contiguous A-units at subsites -2 and -1	Euglena gracilis	?	-	?

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Release 2023.1 (January 2023)