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Literature summary extracted from
- Cloning and functional characterization of two abiotic stress-responsive Jerusalem artichoke (Helianthus tuberosus) fructan 1-exohydrolases (1-FEHs) (2015), Plant Mol. Biol., 87, 81-98 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.80 | expressed in Pichia pastoris X-33 cells | Helianthus tuberosus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.80 | sucrose | 13% inhibition of isoform 1-FEH I at 10 mM and 49% inhibition at 100 mM sucrose; 21% inhibition of isoform 1-FEH II at 10 mM and 65% at 100 mM sucrose | Helianthus tuberosus |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.80 | Helianthus tuberosus | A0A0D5A4E4 | Jerusalem artichoke | - |
| 3.2.1.80 | Helianthus tuberosus | A0A0D5A4L3 | Jerusalem artichoke | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | glycoprotein | isoform 1-FEH I has seven potential N-glycosylation sites | Helianthus tuberosus |
| 3.2.1.80 | glycoprotein | isoform 1-FEH II has four potential N-glycosylation sites | Helianthus tuberosus |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.80 | ammonium sulfate precipitation and Ni-NTA column chromatography | Helianthus tuberosus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.80 | leaf | - |
Helianthus tuberosus | - |
| 3.2.1.80 | stem | - |
Helianthus tuberosus | - |
| 3.2.1.80 | tuber | - |
Helianthus tuberosus | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.80 | 1-kestose + H2O | 100% activity | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | 1F-fructofuranosylnystose + H2O | 98% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | 1F-fructofuranosylnystose + H2O | 99% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | inulin + H2O | 25% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | inulin + H2O | 31% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | levan + H2O | 2% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | levan + H2O | 0.9% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | additional information | inactive with sucrose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | nystose + H2O | 52% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | nystose + H2O | 58% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
| 3.2.1.80 | sucrose + H2O | 0.3% activity compared to 1-kestose | Helianthus tuberosus | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | ? | x * 62800, isoform 1-FEH I, calculated from amino acid sequence | Helianthus tuberosus |
| 3.2.1.80 | ? | x * 65100, isoform 1-FEH II, calculated from amino acid sequence | Helianthus tuberosus |
| 3.2.1.80 | ? | x * 90000, isoform 1-FEH I, SDS-PAGE | Helianthus tuberosus |
| 3.2.1.80 | ? | x * 90000, isoform 1-FEH II, SDS-PAGE | Helianthus tuberosus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.80 | 1-FEH I | isoform | Helianthus tuberosus |
| 3.2.1.80 | 1-FEH II | isoform | Helianthus tuberosus |
| 3.2.1.80 | fructan 1-exohydrolase | - |
Helianthus tuberosus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 35 | - |
- |
Helianthus tuberosus |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 25 | 40 | isoform 1-FEH I shows more than 70% activity between 25 and 40°C | Helianthus tuberosus |
| 3.2.1.80 | 30 | 40 | isoform 1-FEH II shows about 40% activity at 30°C, 100% activity at 35°C and about 75% activity at 40°C | Helianthus tuberosus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 6 | - |
- |
Helianthus tuberosus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.80 | 5 | 6 | isoform 1-FEH I shows more than 80% activity between pH 5.0 and 6.0 | Helianthus tuberosus |
| 3.2.1.80 | 5.5 | 7 | isoform 1-FEH II shows more than 50% activity between pH 5.5 and 7.0 | Helianthus tuberosus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.80 | Helianthus tuberosus | isoform 1-FEH II, calculated from amino acid sequence | - |
4.9 |
| 3.2.1.80 | Helianthus tuberosus | isoform 1-FEH I, calculated from amino acid sequence | - |
6.7 |
Expression
| EC Number | Organism | Comment | Expression |
|---|---|---|---|
| 3.2.1.80 | Helianthus tuberosus | 15% (w/v) PEG treatments slightly inhibit the expression of isoform 1-FEH II in tubers | down |
| 3.2.1.80 | Helianthus tuberosus | isoform 1-FEH I transcripts accumulate to high levels in the developing leaves and stems of artichoke. The level of the isoform transcript is significantly increased in the stems and tubers of 100 mM NaCl-treated plants | up |
| 3.2.1.80 | Helianthus tuberosus | the expression levels of isoform 1-FEH II increase in tubers during tuber sprouting. The level of the isoform transcript is significantly increased in the stems and tubers of 100 mM NaCl-treated plants | up |
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