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Literature summary extracted from

  • Gou, J.; Hao, F.; Huang, C.; Kwon, M.; Chen, F.; Li, C.; Liu, C.; Ro, D.K.; Tang, H.; Zhang, Y.
    Discovery of a non-stereoselective cytochrome P450 catalyzing either 8alpha- or 8beta-hydroxylation of germacrene A acid from the Chinese medicinal plant, Inula hupehensis (2018), Plant J., 93, 92-106 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.168 expression in yeast Inula hupehensis
1.14.14.170 expression in yeast Inula hupehensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 Inula hupehensis the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.170 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 Inula hupehensis the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.168 Inula hupehensis
-
-
-
1.14.14.170 Inula hupehensis
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-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.14.168
-
 Inula hupehensis
1.14.14.170
-
 Inula hupehensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.14.168 flower the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
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1.14.14.168 leaf the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
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1.14.14.168 root the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
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1.14.14.168 stem the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
-
1.14.14.170 flower the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
-
1.14.14.170 leaf the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
-
1.14.14.170 root the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
-
1.14.14.170 stem the highest level of CYP71BL6 transcript is observed in the leaves with relatively lower expression in the flowers, and much lower levels in the stems and roots Inula hupehensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone Inula hupehensis 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.168 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 germacra-1(10),4,11(13)-trien-12-oate i.e. germacrene A acid Inula hupehensis 8beta-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.170 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide. The enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone Inula hupehensis 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.170 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 the product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide Inula hupehensis 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.170 germacra-1(10),4,11(13)-trien-12-oate + [reduced NADPH-hemoprotein reductase] + O2 overall reaction. The product 8alpha-hydroxygermacra-1(10),4,11(13)-trien-12-oate spontaneously lactonizes to 8-epi-inunolide Inula hupehensis 8-epi-inunolide + [oxidized NADPH-hemoprotein reductase] + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.168 CYP71BL6
-
 Inula hupehensis
1.14.14.170 CYP71BL6
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 Inula hupehensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.168 28
-
 assay at Inula hupehensis
1.14.14.170 28
-
 assay at Inula hupehensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.14.168 7.5
-
 assay at Inula hupehensis
1.14.14.170 7.5
-
 assay at Inula hupehensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.168 cytochrome P-450 heme-thiolate protein Inula hupehensis
1.14.14.170 cytochrome P-450 heme-thiolate protein Inula hupehensis

General Information

EC Number General Information Comment Organism
1.14.14.168 metabolism the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone Inula hupehensis
1.14.14.170 metabolism the enzyme is involved in the biosynthesis of C12,8alpha-sesquiterpene lactone Inula hupehensis