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Literature summary extracted from

  • Cardon, F.; Pallisse, R.; Bardor, M.; Caron, A.; Vanier, J.; Ele Ekouna, J.P.; Lerouge, P.; Boitel-Conti, M.; Guillet, M.
    Brassica rapa hairy root based expression system leads to the production of highly homogenous and reproducible profiles of recombinant human alpha-L-iduronidase (2019), Plant Biotechnol. J., 17, 505-516 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.76 expressed in Brassica rapa hairy roots Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.76 0.12
-
 4-methylumbelliferyl alpha-L-iduronide recombinant enzyme, at pH 3.5 and 37°C Homo sapiens
3.2.1.76 0.13
-
 4-methylumbelliferyl alpha-L-iduronide Aldurazyme, at pH 3.5 and 37°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.76 lysosome
-
 Homo sapiens 5764
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.76 Homo sapiens P35475
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.76 glycoprotein the enzyme has 6 N-glycosylation sites Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.76 Eshmuno S column chromatography and Toyopearl phenyl 650 M column chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.76 4-methylumbelliferyl alpha-L-iduronide + H2O
-
 Homo sapiens 4-methylumbelliferone + alpha-L-iduronic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.76 ? x * 71000, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.76 Aldurazyme commercial preparation Homo sapiens
3.2.1.76 alpha-L-iduronidase
-
 Homo sapiens
3.2.1.76 IDUA
-
 Homo sapiens
3.2.1.76 rIDUA_RLT recombinant enzyme Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.76 9.4
-
 4-methylumbelliferyl alpha-L-iduronide Aldurazyme, at pH 3.5 and 37°C Homo sapiens
3.2.1.76 11.8
-
 4-methylumbelliferyl alpha-L-iduronide recombinant enzyme, at pH 3.5 and 37°C Homo sapiens