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Literature summary extracted from
- Molecular cloning and functional analysis of the ortho-hydroxylases of p-coumaroyl coenzyme A/feruloyl coenzyme A involved in formation of umbelliferone and scopoletin in sweet potato, Ipomoea batatas (L.) Lam (2012), Phytochemistry, 74, 49-57 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.11.61 | expression in Escherichia coli | Ipomoea batatas |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.61 | 0.0092 | - |
trans-feruloyl-CoA | pH 6.5, 30°C | Ipomoea batatas |  |
| 1.14.11.61 | 0.0102 | - |
trans-feruloyl-CoA | pH 6.5, 30°C | Ipomoea batatas | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.61 | Fe2+ | absolutely required | Ipomoea batatas | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.61 | Ipomoea batatas | G9M9M1 | isoform F6H1-2 | - |
| 1.14.11.61 | Ipomoea batatas | G9M9M2 | isoform F6H1-3 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.14.11.61 | tuber | - |
Ipomoea batatas | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.61 | caffeoyl-CoA + 2-oxoglutarate + O2 | - |
Ipomoea batatas | 6'-hydroxycaffeoyl-CoA + succinate + CO2 | - |
? | |
| 1.14.11.61 | additional information | no substrates: cinnamoyl-CoA and the respective free acids | Ipomoea batatas | ? | - |
? | |
| 1.14.11.61 | trans-feruloyl-CoA + 2-oxoglutarate + O2 | - |
Ipomoea batatas | trans-6-hydroxyferuloyl-CoA + succinate + CO2 | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.11.61 | ? | x * 43000, SDS-PAGE | Ipomoea batatas |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.11.61 | F6H1-2 | - |
Ipomoea batatas |
| 1.14.11.61 | F6H1-3 | - |
Ipomoea batatas |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.61 | 2.68 | - |
trans-feruloyl-CoA | pH 6.5, 30°C | Ipomoea batatas | |
| 1.14.11.61 | 2.84 | - |
trans-feruloyl-CoA | pH 6.5, 30°C | Ipomoea batatas | |
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Release 2023.1 (January 2023)
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