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Literature summary extracted from

  • Igetei, J.E.; Liddell, S.; El-Faham, M.; Doenhoff, M.J.
    Purification of a chymotrypsin-like enzyme present on adult Schistosoma mansoni worms from infected mice and its characterization as a host carboxylesterase (2016), Parasitology, 143, 646-657 .
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.1 bis-4-nitrophenyl phosphate BNPP Mus musculus
3.1.1.1 bis-4-nitrophenyl phosphate BNPP Rattus norvegicus
3.1.1.1 PMSF
-
 Mus musculus
3.1.1.1 PMSF
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.1 extracellular the enzyme is present in soluble form and on the surface of adult Schistosoma mansoni worms in infected mice Mus musculus
-
-
3.1.1.1 extracellular the enzyme is soluble Rattus norvegicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.1 Mus musculus P23953 random-bred mice, infected with Schistosoma mansoni
-
3.1.1.1 Rattus norvegicus P10959
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.1 purification of a host chymotrypsin-like enzyme present on adult Schistosoma mansoni worms from infected mice, purification of the chymotrypsin-like enzyme from mouse blood plasma by SDS-PAGE Mus musculus
3.1.1.1 purification of the chymotrypsin-like enzyme from rat blood plasma by SDS-PAGE Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.1 blood plasma
-
 Mus musculus
-
3.1.1.1 blood plasma
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.1 alpha-naphthyl acetate + H2O
-
 Mus musculus alpha-naphthol + acetate
-
 ?
3.1.1.1 alpha-naphthyl acetate + H2O
-
 Rattus norvegicus alpha-naphthol + acetate
-
 ?
3.1.1.1 beta-naphthyl acetate + H2O
-
 Mus musculus beta-naphthol + acetate
-
 ?
3.1.1.1 beta-naphthyl acetate + H2O
-
 Rattus norvegicus beta-naphthol + acetate
-
 ?
3.1.1.1 N-acetyl-DL-phenylalanine beta-naphthyl ester + H2O NAPBNE, a chromogenic substrate of chymotrypsin-like enzymes Mus musculus N-acetyl-DL-phenylalanine + beta-naphthol
-
 ?
3.1.1.1 N-acetyl-DL-phenylalanine beta-naphthyl ester + H2O NAPBNE, a chromogenic substrate of chymotrypsin-like enzymes Rattus norvegicus N-acetyl-DL-phenylalanine + beta-naphthol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.1 ? x * 85000, about, SDS-PAGE Mus musculus
3.1.1.1 ? x * 85000, about, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.1.1 carboxylesterase 1C
-
 Mus musculus
3.1.1.1 carboxylesterase 1C
-
 Rattus norvegicus
3.1.1.1 CES 1C
-
 Mus musculus
3.1.1.1 Est1C
-
 Mus musculus
3.1.1.1 Est1C
-
 Rattus norvegicus
3.1.1.1 serine protease-like
-
 Mus musculus
3.1.1.1 serine protease-like
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.1 37
-
 assay at Mus musculus
3.1.1.1 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.1 7.4
-
 assay at Mus musculus
3.1.1.1 7.4
-
 assay at Rattus norvegicus

General Information

EC Number General Information Comment Organism
3.1.1.1 physiological function possible role of the enzyme in the mouse host-parasite relationship might be to ease the passage of worms through the host's blood vessels and/or in immune evasion. Immunofluorescent detection of the host-derived enzyme on adult worms Mus musculus