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Literature summary extracted from

  • Beck, K.; Lipps, G.
    Properties of an unusual DNA primase from an archaeal plasmid (2007), Nucleic Acids Res., 35, 5635-5645 .
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.7.102 ATP presence of ATP is required Sulfolobus islandicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.102 expression in Escherichia coli Sulfolobus islandicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.102 0.032
-
 dATP 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus
2.7.7.102 0.15
-
 ATP 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus
2.7.7.102 0.2
-
 5'-CTTCTTCTGTGC-3' 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.102 Sulfolobus islandicus Q54324 encoded on plasmid pRN1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.102 5'-CTTCTTCTGTGC-3' + n dNTP i.e. minimal substrate which supports synthesis of the full-length primer. The base 3' to the central GTG motif is critical for primer synthesis and the bases 5' of the GTG determine the length of the primer or run-off product Sulfolobus islandicus 5'-CTTCTTCTGTGC-3'/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 M13 ssDNA + n ATP
-
 Sulfolobus islandicus M13 ssDNA/pppN(pA)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 M13 ssDNA + n dATP
-
 Sulfolobus islandicus M13 ssDNA/pppdN(pdA)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 M13 ssDNA + n dNTP
-
 Sulfolobus islandicus M13 ssDNA/pppdN(pdN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 M13 ssDNA + n NTP
-
 Sulfolobus islandicus M13 ssDNA/pppN(pN)n-1 + (n-1) diphosphate
-
 ?
2.7.7.102 additional information homopolymers as substrates do not yield detectable primase activity Sulfolobus islandicus ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.7.102 0.1
-
 5'-CTTCTTCTGTGC-3' 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus
2.7.7.102 0.11
-
 ATP 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus
2.7.7.102 0.16
-
 dATP 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus

General Information

EC Number General Information Comment Organism
2.7.7.102 physiological function the primase activity synthesizes a mixed primer consisting of a single ribonucleotide at the 5' end followed by seven deoxynucleotides. Ribonucleotides and deoxynucleotides are strictly required at the respective positions within the primer. The primase activity is highly sequence-specific and requires the trinucleotide motif GTG in the template. Primer synthesis starts outside of the recognition motif, immediately 5' to the recognition motif. Non-complementary bases are not incorporated into the primer Sulfolobus islandicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.7.7.102 0.014
-
 5'-CTTCTTCTGTGC-3' 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus
2.7.7.102 0.016
-
 ATP 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus
2.7.7.102 0.023
-
 dATP 50°C, pH 7.5, synthesis of full-length primer Sulfolobus islandicus