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Literature summary extracted from

  • Verma, R.; Reichermeier, K.M.; Burroughs, A.M.; Oania, R.S.; Reitsma, J.M.; Aravind, L.; Deshaies, R.J.
    Vms1 and ANKZF1 peptidyl-tRNA hydrolases release nascent chains from stalled ribosomes (2018), Nature, 557, 446-451 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.29 gene ANKZF1, transient recombinant expression of His-tagged wild-type and mutant enzymes in HEK-293 cells Homo sapiens
3.1.1.29 gene VMS1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.29 Q246L site-directed mutagenesis, inactive active site mutant Homo sapiens
3.1.1.29 Q295L site-directed mutagenesis, inactive mutant Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.29 peptidyl-tRNA + H2O Saccharomyces cerevisiae
-
 peptide + tRNA
-
 ?
3.1.1.29 peptidyl-tRNA + H2O Homo sapiens
-
 peptide + tRNA
-
 ?
3.1.1.29 peptidyl-tRNA + H2O Saccharomyces cerevisiae ATCC 204508
-
 peptide + tRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.29 Homo sapiens Q9H8Y5
-
-
3.1.1.29 Saccharomyces cerevisiae Q04311
-
-
3.1.1.29 Saccharomyces cerevisiae ATCC 204508 Q04311
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.29 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Saccharomyces cerevisiae
3.1.1.29 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.29 Flag-tagged CRPNSKn-tRNA + H2O
-
 Saccharomyces cerevisiae Flag-tagged CRPNSKn + tRNA
-
 ?
3.1.1.29 Flag-tagged CRPNSKn-tRNA + H2O purified recombinant Ankzf1 catalyzes deacylation Homo sapiens Flag-tagged CRPNSKn + tRNA
-
 ?
3.1.1.29 Flag-tagged CRPNSKn-tRNA + H2O
-
 Saccharomyces cerevisiae ATCC 204508 Flag-tagged CRPNSKn + tRNA
-
 ?
3.1.1.29 peptidyl-tRNA + H2O
-
 Saccharomyces cerevisiae peptide + tRNA
-
 ?
3.1.1.29 peptidyl-tRNA + H2O
-
 Homo sapiens peptide + tRNA
-
 ?
3.1.1.29 peptidyl-tRNA + H2O
-
 Saccharomyces cerevisiae ATCC 204508 peptide + tRNA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.29 Ankzf1
-
 Homo sapiens
3.1.1.29 Cdc48 adaptor
-
 Saccharomyces cerevisiae
3.1.1.29 peptidyl-tRNA hydrolase
-
 Saccharomyces cerevisiae
3.1.1.29 peptidyl-tRNA hydrolase
-
 Homo sapiens
3.1.1.29 Vms1
-
 Saccharomyces cerevisiae
3.1.1.29 ZNF744
-
 Homo sapiens

General Information

EC Number General Information Comment Organism
3.1.1.29 evolution Ankzf1 is Saccharomyces cerevisiae Vms1 homologue Homo sapiens
3.1.1.29 evolution Vms1 is a member of a clade of eRF1 homologues that is designated the Vms1-like RF1 clade (VLRF1) Saccharomyces cerevisiae
3.1.1.29 metabolism enzyme Vms1, which contains a Cdc48-binding VIM motif, is implicated in the ribosome quality control (RQC) pathway. The 60S subunits retain the peptidyl-tRNA nascent chains, which recruit the RQC complex comprised of Rqc1-Rqc2-Ltn1-Cdc48-Ufd1-Npl4. Nascent chains ubiquitylated by the E3/ubiquitin ligase Ltn1 are extracted from the 60S by the ATPase Cdc48-Ufd1-Npl4 and presented to the 26S proteasome for degradation. The Vms1-dependent reaction does not display strong dependence on substrate ubiquitylation Saccharomyces cerevisiae
3.1.1.29 additional information enzyme Vms1 activity is dependent on a conserved catalytic glutamine. The putative catalytic region of Vms1 and other VLRF1 proteins is flanked by additional N- and C-terminal domains. The N-terminal C2H2-zinc finger of Vms1 is specifically related to those of Rei1 and certain SBDS paralogues, which function at late steps in 60S subunit maturation Saccharomyces cerevisiae
3.1.1.29 physiological function Ankzf1 is a peptidyl-tRNA hydrolase Homo sapiens
3.1.1.29 physiological function the Cdc48 adaptor Vms1 is a peptidyl-tRNA hydrolase. Yeast Cdc48, a AAA+ ATPase that is conserved across eukaryotes and archaea, is a protein unfoldase that engages in myriad cellular functions through binding of adaptors such as Ufd1-Npl4 (UN), which bind both Cdc48 and ubiquitylated substrate proteins Saccharomyces cerevisiae