Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kant, S.; Agarwal, S.; Pancholi, P.; Pancholi, V.
    The Streptococcus pyogenes orphan protein tyrosine phosphatase, SP-PTP, possesses dual specificity and essential virulence regulatory functions (2015), Mol. Microbiol., 97, 515-540 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.16 gene SPy_0036, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Streptococcus pyogenes serotype M28
3.1.3.16 gene SPy_0039, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Streptococcus pyogenes serotype M1
3.1.3.48 gene SPy_0036, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Streptococcus pyogenes serotype M28
3.1.3.48 gene SPy_0039, quantitative real time PCR expression analysis, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli Streptococcus pyogenes serotype M1

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.16 C8A site-directed mutagenesis, inactive enzyme mutant of SP-PTP Streptococcus pyogenes serotype M1
3.1.3.16 additional information A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of a M1T1DELTAPTP enzyme knockout mutant, phenotype, overview Streptococcus pyogenes serotype M1
3.1.3.16 additional information A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of Streptoccocus pyogenes mutant lacking the ptp-(Spy_0036) gene derived from the Type M1 M1T15448 strain by allelic replacement Streptococcus pyogenes serotype M28
3.1.3.16 R14A site-directed mutagenesis, the enzyme mutant of SP-PTP shows highly reduced activity compared to wild-type Streptococcus pyogenes serotype M1
3.1.3.48 C8A site-directed mutagenesis, inactive enzyme mutant of SP-PTP Streptococcus pyogenes serotype M1
3.1.3.48 additional information A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of a M1T1DELTAPTP enzyme knockout mutant, phenotype, overview Streptococcus pyogenes serotype M1
3.1.3.48 additional information A-549 human lung cells infected with group A Streptococcus (GAS) mutants lacking enzyme SP-PTP display increased Ser-/Thr-/Tyr-phosphorylation. Generation of Streptoccocus pyogenes mutant lacking the ptp-(Spy_0036) gene derived from the Type M1 M1T15448 strain by allelic replacement Streptococcus pyogenes serotype M28
3.1.3.48 R14A site-directed mutagenesis, the enzyme mutant of SP-PTP shows highly reduced activity compared to wild-type Streptococcus pyogenes serotype M1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.16 additional information
-
 additional information Michaelis-Menten and Lineweaver-Burk kinetics Streptococcus pyogenes serotype M1
3.1.3.16 additional information
-
 additional information Michaelis-Menten and Lineweaver-Burk kinetics Streptococcus pyogenes serotype M28
3.1.3.48 additional information
-
 additional information Michaelis-Menten and Lineweaver-Burk kinetics Streptococcus pyogenes serotype M1
3.1.3.48 additional information
-
 additional information Michaelis-Menten and Lineweaver-Burk kinetics Streptococcus pyogenes serotype M28

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.16 extracellular the enzyme is secreted Streptococcus pyogenes serotype M1
-
-
3.1.3.48 extracellular the enzyme is secreted Streptococcus pyogenes serotype M1
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.16 [a protein]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M1
-
 [a protein]-serine/threonine + phosphate
-
 ?
3.1.3.16 [a protein]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M28
-
 [a protein]-serine/threonine + phosphate
-
 ?
3.1.3.16 [SP-STK kinase]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M1
-
 [SP-STK kinase]-serine/threonine + phosphate
-
 ?
3.1.3.16 [SP-STK kinase]-serine/threonine phosphate + H2O Streptococcus pyogenes serotype M28
-
 [SP-STK kinase]-serine/threonine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Streptococcus pyogenes serotype M1
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Streptococcus pyogenes serotype M28
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O Streptococcus pyogenes serotype M28 MGAS6180
-
 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [epidermal growth factor receptor]-tyrosine phosphate + H2O Streptococcus pyogenes serotype M28
-
 [epidermal growth factor receptor]-tyrosine + phosphate
-
 ?
3.1.3.48 [epidermal growth factor receptor]-tyrosine phosphate + H2O Streptococcus pyogenes serotype M1 enzyme SP-PTP dephosphorylates the activated EGFR (170 kDa protein migrating at about 240 kDa) present in MDA-MB-231 cell lines [epidermal growth factor receptor]-tyrosine + phosphate
-
 ?
3.1.3.48 [epidermal growth factor receptor]-tyrosine phosphate + H2O Streptococcus pyogenes serotype M28 MGAS6180
-
 [epidermal growth factor receptor]-tyrosine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.16 Streptococcus pyogenes serotype M1 Q9A1Y0 MGAS5005 genome
-
3.1.3.16 Streptococcus pyogenes serotype M28 Q48VV6 M1SF370 genome
-
3.1.3.16 Streptococcus pyogenes serotype M28 MGAS6180 Q48VV6 M1SF370 genome
-
3.1.3.48 Streptococcus pyogenes serotype M1 Q9A1Y0 MGAS5005 genome
-
3.1.3.48 Streptococcus pyogenes serotype M28 Q48VV6 M1SF370 genome
-
3.1.3.48 Streptococcus pyogenes serotype M28 MGAS6180 Q48VV6 M1SF370 genome
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.16 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Streptococcus pyogenes serotype M1
3.1.3.16 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Streptococcus pyogenes serotype M28
3.1.3.48 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Streptococcus pyogenes serotype M1
3.1.3.48 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Streptococcus pyogenes serotype M28

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Streptococcus pyogenes serotype M1 4-nitrophenol + phosphate
-
 ?
3.1.3.16 4-nitrophenyl phosphate + H2O
-
 Streptococcus pyogenes serotype M28 4-nitrophenol + phosphate
-
 ?
3.1.3.16 additional information SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M1 ?
-
 ?
3.1.3.16 additional information SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M28 ?
-
 ?
3.1.3.16 additional information SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M28 MGAS6180 ?
-
 ?
3.1.3.16 [a protein]-serine/threonine phosphate + H2O
-
 Streptococcus pyogenes serotype M1 [a protein]-serine/threonine + phosphate
-
 ?
3.1.3.16 [a protein]-serine/threonine phosphate + H2O
-
 Streptococcus pyogenes serotype M28 [a protein]-serine/threonine + phosphate
-
 ?
3.1.3.16 [SP-STK kinase]-serine/threonine phosphate + H2O
-
 Streptococcus pyogenes serotype M1 [SP-STK kinase]-serine/threonine + phosphate
-
 ?
3.1.3.16 [SP-STK kinase]-serine/threonine phosphate + H2O
-
 Streptococcus pyogenes serotype M28 [SP-STK kinase]-serine/threonine + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Streptococcus pyogenes serotype M1 4-nitrophenol + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Streptococcus pyogenes serotype M28 4-nitrophenol + phosphate
-
 ?
3.1.3.48 4-nitrophenyl phosphate + H2O
-
 Streptococcus pyogenes serotype M28 MGAS6180 4-nitrophenol + phosphate
-
 ?
3.1.3.48 additional information enzyme SP-PTP does not dephosphorylate the C-terminal fragment of EGFR with intact catalytic domain in vitro. SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M1 ?
-
 ?
3.1.3.48 additional information enzyme SP-PTP does not dephosphorylate the C-terminal fragment of EGFR with intact catalytic domain in vitro. SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M28 ?
-
 ?
3.1.3.48 additional information enzyme SP-PTP does not dephosphorylate the C-terminal fragment of EGFR with intact catalytic domain in vitro. SP-PTP possesses a tyrosine phosphatase activity that varies based on the type of substrates and reaction conditions. Enzyme SP-PTP also possesses Ser/Thr phosphatase activity dephosphorylating the autophosphorylated SP-STKK, i.e. the kinase domain of kinase SP-STK Streptococcus pyogenes serotype M28 MGAS6180 ?
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Streptococcus pyogenes serotype M1 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Streptococcus pyogenes serotype M28 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [a protein]-tyrosine phosphate + H2O
-
 Streptococcus pyogenes serotype M28 MGAS6180 [a protein]-tyrosine + phosphate
-
 ?
3.1.3.48 [epidermal growth factor receptor]-tyrosine phosphate + H2O
-
 Streptococcus pyogenes serotype M1 [epidermal growth factor receptor]-tyrosine + phosphate
-
 ?
3.1.3.48 [epidermal growth factor receptor]-tyrosine phosphate + H2O
-
 Streptococcus pyogenes serotype M28 [epidermal growth factor receptor]-tyrosine + phosphate
-
 ?
3.1.3.48 [epidermal growth factor receptor]-tyrosine phosphate + H2O enzyme SP-PTP dephosphorylates the activated EGFR (170 kDa protein migrating at about 240 kDa) present in MDA-MB-231 cell lines Streptococcus pyogenes serotype M1 [epidermal growth factor receptor]-tyrosine + phosphate
-
 ?
3.1.3.48 [epidermal growth factor receptor]-tyrosine phosphate + H2O
-
 Streptococcus pyogenes serotype M28 MGAS6180 [epidermal growth factor receptor]-tyrosine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.16 ? x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE Streptococcus pyogenes serotype M1
3.1.3.16 ? x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE Streptococcus pyogenes serotype M28
3.1.3.48 ? x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE Streptococcus pyogenes serotype M1
3.1.3.48 ? x * 16954, sequence calculation, x * 19000, recombinant enzyme, SDS-PAGE Streptococcus pyogenes serotype M28

Synonyms

EC Number Synonyms Comment Organism
3.1.3.16 More cf. EC 3.1.3.48 Streptococcus pyogenes serotype M1
3.1.3.16 More cf. EC 3.1.3.48 Streptococcus pyogenes serotype M28
3.1.3.16 SP-PTP
-
 Streptococcus pyogenes serotype M1
3.1.3.16 SPy_0036
-
 Streptococcus pyogenes serotype M28
3.1.3.16 SPy_0039
-
 Streptococcus pyogenes serotype M1
3.1.3.48 LMW PTPase
-
 Streptococcus pyogenes serotype M1
3.1.3.48 LMW PTPase
-
 Streptococcus pyogenes serotype M28
3.1.3.48 low-molecular weight PTPase
-
 Streptococcus pyogenes serotype M1
3.1.3.48 low-molecular weight PTPase
-
 Streptococcus pyogenes serotype M28
3.1.3.48 More cf. EC 3.1.3.16 Streptococcus pyogenes serotype M1
3.1.3.48 More cf. EC 3.1.3.16 Streptococcus pyogenes serotype M28
3.1.3.48 orphan protein tyrosine phosphatase
-
 Streptococcus pyogenes serotype M1
3.1.3.48 orphan protein tyrosine phosphatase
-
 Streptococcus pyogenes serotype M28
3.1.3.48 SP-PTP
-
 Streptococcus pyogenes serotype M1
3.1.3.48 SPy_0036
-
 Streptococcus pyogenes serotype M28
3.1.3.48 SPy_0039
-
 Streptococcus pyogenes serotype M1

Expression

EC Number Organism Comment Expression
3.1.3.16 Streptococcus pyogenes serotype M1 the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell up
3.1.3.16 Streptococcus pyogenes serotype M28 the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell up
3.1.3.48 Streptococcus pyogenes serotype M1 the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell up
3.1.3.48 Streptococcus pyogenes serotype M28 the expression of SP-PTP is highly upregulated in the group A Streptococcus population within the host cell up

General Information

EC Number General Information Comment Organism
3.1.3.16 evolution group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) Streptococcus pyogenes serotype M1
3.1.3.16 evolution group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) Streptococcus pyogenes serotype M28
3.1.3.16 malfunction absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morphology, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype Streptococcus pyogenes serotype M1
3.1.3.16 malfunction absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morphology, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype Streptococcus pyogenes serotype M28
3.1.3.16 physiological function SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence Streptococcus pyogenes serotype M1
3.1.3.16 physiological function SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence Streptococcus pyogenes serotype M28
3.1.3.48 evolution group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) Streptococcus pyogenes serotype M1
3.1.3.48 evolution group A Streptococcus (GAS) Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. GAS lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). SP-PTP falls into the category of low-molecular weight PTPases (LMW PTPases) Streptococcus pyogenes serotype M28
3.1.3.48 malfunction absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morpholog, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype Streptococcus pyogenes serotype M1
3.1.3.48 malfunction absence of SP-PTP and SP-PTP enzymatic activity adversely affects GAS cell morpholog, the slow growth of M1T1DELTAPTP is associated with defective cell divisiony. The GAS mutant lacking SP-PTP poorly adheres to and invades human respiratory cells and affects in vivo phosphorylation events in human respiratory cells, phenotype Streptococcus pyogenes serotype M28
3.1.3.48 physiological function SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence Streptococcus pyogenes serotype M1
3.1.3.48 physiological function SP-PTP is a secretory protein tyrosine phosphatase and plays a crucial role in group A Streptococcus (GAS) growth. As a group A Streptococcus, Streptococcus pyogenes is a human pathogen that causes high morbidity and mortality. It lacks a gene encoding tyrosine kinase but contains one encoding tyrosine phosphatase (SP-PTP). Enzymatically active SP-PTP plays a crucial role in cell division, the function of SP-PTP is physiologically relevant for GAS growth and cell division. SP-PTP is essential for GAS virulence Streptococcus pyogenes serotype M28