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Literature summary extracted from

  • Zhang, S.; Ma, G.; Liu, Y.; Ling, B.
    Theoretical study of the hydrolysis mechanism of 2-pyrone-4,6-dicarboxylate (PDC) catalyzed by LigI (2015), J. Mol. Graph. Model., 61, 21-29 .
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.57 Sphingobium sp. O87170
-
-
3.1.1.57 Sphingobium sp. NBRC 103272 O87170
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.57 2-oxo-2H-pyran-4,6-dicarboxylate + H2O
-
 Sphingobium sp. (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
-
 ?
3.1.1.57 2-oxo-2H-pyran-4,6-dicarboxylate + H2O
-
 Sphingobium sp. NBRC 103272 (1E)-4-oxobut-1-ene-1,2,4-tricarboxylate
-
 ?
3.1.1.57 additional information the catalytic reaction contains the nucleophilic attack, the cleavage of C-O bond of lactone (substrate) and the intramolecular proton transfer. The intermediate has two intramolecular proton transfer pathways, leading to two final hydrolysis products. According to the energy profile, 4-carboxy-2-hydroxymuconate is the main hydrolysis product, and the isomerization between 4-carboxy-2-hydroxymuconate and 4-oxalomesaconate is suggested to occur in solvent. Residue Asp248 acts as a general base to activate the hydrolytic water molecule. Residues His31, His33 and His180 play assistant roles Sphingobium sp. ?
-
 ?
3.1.1.57 additional information the catalytic reaction contains the nucleophilic attack, the cleavage of C-O bond of lactone (substrate) and the intramolecular proton transfer. The intermediate has two intramolecular proton transfer pathways, leading to two final hydrolysis products. According to the energy profile, 4-carboxy-2-hydroxymuconate is the main hydrolysis product, and the isomerization between 4-carboxy-2-hydroxymuconate and 4-oxalomesaconate is suggested to occur in solvent. Residue Asp248 acts as a general base to activate the hydrolytic water molecule. Residues His31, His33 and His180 play assistant roles Sphingobium sp. NBRC 103272 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.57 LigI
-
 Sphingobium sp.