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Literature summary extracted from

  • Emampour, M.; Noghabi, K.; Zahiri, H.
    Molecular cloning and biochemical characterization of a novel cold-adapted alpha-amylase with multiple extremozyme characteristics (2015), J. Mol. Catal. B, 111, 79-86 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.1 glycerol activates 15% at 20% v/v, and 27% at 50% v/v Exiguobacterium sp. SH3
3.2.1.1 additional information no or poor effects by glucose, sucrose, maltose, mannitol, trehalose, sorbitol, myo-inositol, and iodoacetate at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 Triton X-100 activates 33% at 20% v/v, and 46% at 50% v/v Exiguobacterium sp. SH3
3.2.1.1 Tween 20 activates 27% at 20% v/v, and 39% at 50% v/v Exiguobacterium sp. SH3

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 gene amy-E, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS Exiguobacterium sp. SH3

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 (NH4)2SO4 slight inhibition at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 acetone inhibits 34% at 20% v/v, and 61% at 50% v/v Exiguobacterium sp. SH3
3.2.1.1 butanol inhibits 15% at 20% v/v, and 21% at 50% v/v Exiguobacterium sp. SH3
3.2.1.1 DMSO inhibits 20% at 20% v/v, and 31% at 50% v/v Exiguobacterium sp. SH3
3.2.1.1 EDTA slight inhibition at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 ethanol inhibits 9% at 20% v/v, and 18% at 50% v/v Exiguobacterium sp. SH3
3.2.1.1 guanidinium hydrochloride slight inhibition at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 methanol inhibits 7% at 20% v/v, and 13% at 50% v/v Exiguobacterium sp. SH3
3.2.1.1 additional information no or poor effects by glucose, sucrose, maltose, mannitol, trehalose, sorbitol, myo-inositol, and iodoacetate at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 SDS slight inhibition at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 Urea slight inhibition at 5-10 mM Exiguobacterium sp. SH3

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information Km of soluble starch hydrolysis is 2.29 mg/ml, thermodynamics, overview Exiguobacterium sp. SH3

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular the enzyme has a signal peptide of 25 amino acids at the N-terminus Exiguobacterium sp. SH3
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 K+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 Li+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 Mg2+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 additional information no or poor effects by 5-10 mM of Mn2+, Cu2+, Zn2+, and Fe2+ Exiguobacterium sp. SH3
3.2.1.1 Na+ slightly activating at 5-10 mM Exiguobacterium sp. SH3
3.2.1.1 NaCl Amy-E not only is halotolerant but also its activity is stimulated at high salt concentrations in the range of 1-5 M, activates 20% at 2 M Exiguobacterium sp. SH3

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 53000
-
 about, native PAGE, recombinant enzyme Exiguobacterium sp. SH3

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Exiguobacterium sp. SH3 A0A0A0WA73 isolated from soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Exiguobacterium sp. SH3

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O 62.2% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?
3.2.1.1 maltodextrin + H2O 87.6% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?
3.2.1.1 rice flour + H2O 48.7% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?
3.2.1.1 soluble starch + H2O best substrate Exiguobacterium sp. SH3 ?
-
 ?
3.2.1.1 wheat flour + H2O 53.0% activity compared to soluble starch Exiguobacterium sp. SH3 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 53000, recombinant His-tagged enzyme, SDS-PAGE Exiguobacterium sp. SH3

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 Amy-E
-
 Exiguobacterium sp. SH3

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 30
-
 recombinant enzyme Exiguobacterium sp. SH3

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1
-
 80 41% of maximal activity at 0°C, 63%, 50%, 71%, 48%, 39%, 26%, and 14% at 20°C, 10°C, 40°C, 50°C, 60°C, 70°C, and 80°C, respectively Exiguobacterium sp. SH3

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1
-
 60 purified recombinant His-tagged enzyme, stable up to 30°C, the enzyme activity drops to 82%, 39%, and 16% after5 min at 40°C, 50°C, and 60°C, and to 41%, 15%, and 4% after 30 min, respectively Exiguobacterium sp. SH3

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6.5
-
 recombinant enzyme Exiguobacterium sp. SH3

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.1 4 9 activity range, profile overview Exiguobacterium sp. SH3

General Information

EC Number General Information Comment Organism
3.2.1.1 evolution the enzyme belongs to the glycosyl hydrolase family 13, GH13 Exiguobacterium sp. SH3
3.2.1.1 additional information the catalytic triad is formed by residues D210,E239, and D307 Exiguobacterium sp. SH3