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Literature summary extracted from

  • Park, C.; Kim, Y.H.; Lee, S.R.; Park, S.; Jung, Y.; Lee, Y.; Kim, J.S.; Eom, T.; Kim, J.S.; Lee, D.M.; Song, B.S.; Sim, B.W.; Kim, S.U.; Chang, K.T.; Kim, E.
    Characterization of recombinant bovine sperm hyaluronidase and identification of an important Asn-X-Ser/Thr motif for its activity (2018), J. Microbiol. Biotechnol., 28, 1547-1553 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.35 the bovine SPAM1 open reading frame is cloned into the mammalian expression vector pCXN2 and then transfected to the HEK293 cell line Bos taurus

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.35 additional information mutagenesis analysis reveales that among the five potential N-linked glycosylation sites, three contributed to significant inhibition of hyaluronidase activity Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 Bos taurus Q2YDK3
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 semen
-
 Bos taurus
-
3.2.1.35 spermatozoon
-
 Bos taurus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronic acid + H2O the Asn-X-Ser/Thr sequence is essential for this activity Bos taurus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 SPAM1
-
 Bos taurus
3.2.1.35 sperm hyaluronidase 1
-
 Bos taurus