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Literature summary extracted from
- The purification and properties of two isofunctional 2-hydroxy-6-oxohepta-2,4-dienoate hydrolases from Alcaligenes eutrophus strain 345 (1987), J. Gen. Microbiol., 133, 815-822 .
No PubMed abstract available
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.7.1.25 | additional information | EDTA shows no significant stimulatory effect | Cupriavidus necator |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.7.1.25 | Cu2+ | - |
Cupriavidus necator |  |
| 3.7.1.25 | Mn2+ | the activity of isoform HODHI is decreased by about 50% in the presence of Mn2+ | Cupriavidus necator | |
| 3.7.1.25 | additional information | EDTA shows no significant inhibitory effect | Cupriavidus necator | |
| 3.7.1.25 | p-chloromercuribenzoate | isoform HODHI is inactivated to a much greater extent (90%) by p-chloromercuribenzoate than is isoform HODHII (35%) | Cupriavidus necator | |
| 3.7.1.25 | Zn2+ | - |
Cupriavidus necator | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.7.1.25 | 0.0012 | - |
2-hydroxymuconic semialdehyde | isoform HODHI, at pH 7.6 and 23°C | Cupriavidus necator | |
| 3.7.1.25 | 0.058 | - |
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate | isoform HODHI, at pH 7.6 and 23°C | Cupriavidus necator | |
| 3.7.1.25 | 0.36 | - |
(2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate | isoform HODHII, at pH 7.6 and 23°C | Cupriavidus necator | |
| 3.7.1.25 | 62 | - |
2-hydroxymuconic semialdehyde | isoform HODHII, at pH 7.6 and 23°C | Cupriavidus necator | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.7.1.25 | Mn2+ | isoform HODHII activity is stimulated to a level about 50% higher when Mn2+ is present | Cupriavidus necator | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.25 | 104000 | - |
isoform HODHI, gel filtration | Cupriavidus necator |
| 3.7.1.25 | 116000 | - |
isoform HODHII, gel filtration | Cupriavidus necator |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.25 | (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | Cupriavidus necator | - |
(2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
| 3.7.1.25 | (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | Cupriavidus necator ATCC 17707 | - |
(2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.7.1.25 | Cupriavidus necator | - |
- |
- |
| 3.7.1.25 | Cupriavidus necator ATCC 17707 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.7.1.25 | ammonium sulfate precipitation, DEAE-cellulose column chromatography, Sephadex G200 gel filtration, DEAE Sepharose column chromatography, and Sephadex G-150 gel filtration (isoform HODHI only) | Cupriavidus necator |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.7.1.25 | (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Cupriavidus necator | (2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
| 3.7.1.25 | (2Z,4E)-2-hydroxy-6-oxohepta-2,4-dienoate + H2O | - |
Cupriavidus necator ATCC 17707 | (2Z)-2-hydroxypenta-2,4-dienoate + acetate | - |
? | |
| 3.7.1.25 | 2-hydroxy-5-methylmuconic semialdehyde + H2O | - |
Cupriavidus necator | ? | - |
? | |
| 3.7.1.25 | 2-hydroxy-5-methylmuconic semialdehyde + H2O | - |
Cupriavidus necator ATCC 17707 | ? | - |
? | |
| 3.7.1.25 | 2-hydroxymuconic semialdehyde + H2O | - |
Cupriavidus necator | ? | - |
? | |
| 3.7.1.25 | 2-hydroxymuconic semialdehyde + H2O | - |
Cupriavidus necator ATCC 17707 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.7.1.25 | homotrimer | 3 * 34400, isoform HODHI, SDS-PAGE | Cupriavidus necator |
| 3.7.1.25 | homotrimer | 3 * 36700, isoform HODHII, SDS-PAGE | Cupriavidus necator |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.7.1.25 | HOD hydrolase | - |
Cupriavidus necator |
| 3.7.1.25 | HODHI | isoform | Cupriavidus necator |
| 3.7.1.25 | HODHII | isoform | Cupriavidus necator |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.25 | 45 | - |
no activity of isoforms HODHII is present after treatment for 2 min at 45°C and isoform HODHI is inactivated after 4 min at the same temperature | Cupriavidus necator |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.25 | 8.6 | - |
- |
Cupriavidus necator |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.7.1.25 | 5 | 10 | both isoforms are active between pH 5.0 and 10.0. There is a marked decrease in activity above pH 9.0. At pH 7.6, the activity of isoform HODHI and HODHII is about 75-80% of that at pH 8.6 | Cupriavidus necator |
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